STAT5 A/B antibody (pSer726, pSer731)

Catalog No. ABIN7224522

This Rabbit Polyclonal antibody specifically detects STAT5 A/B in WB, ELISA, IF and IHC (p). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for STAT5 A/B antibody (pSer726, pSer731) (ABIN7224522)

Target: STAT5 A/B

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This STAT5 A/B antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-STAT5 A/B Antibody

Binding Specificity: pSer726, pSer731

Purpose: Stat5a/b (phospho Ser726/731) Polyclonal Antibody

Specificity: Phospho-Stat5a/b (S726/731) Polyclonal Antibody detects endogenous levels of Stat5a/b protein only when phosphorylated at S726/731.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human Stat5a/b Phospho-Ser726/731

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:20000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for STAT5 A/B

Target: STAT5 A/B

Alternative Name: Stat5a/b

Background: Rabbit Anti-Stat5a/b (phospho Ser726/731) Polyclonal Antibody,STAT5A, STAT5, Signal transducer and activator of transcription 5A, STAT5B, Signal transducer and activator of transcription 5B,The protein encoded by STAT5A (signal transducer and activator of transcription 5A) is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for tumorigenesis. The mouse counterpart of STAT5A is found to induce the expression of BCL2L1/BCL-X (L), which suggests the antiapoptotic function of this gene in cells. Alternatively spliced transcript variants have been found for STAT5A.,Signal transducer and activator of transcription 5A

Molecular Weight: observerd band 91kDa

Gene ID: 6776, 6777