STON1 antibody (AA 660-740)

Catalog No. ABIN7219210

The Rabbit Polyclonal anti-STON1 antibody is suitable to detect STON1 in samples from Human. It has been validated for WB, ELISA, IF and IHC (p).

Quick Overview for STON1 antibody (AA 660-740) (ABIN7219210)

Target: STON1 (Stonin 1 (STON1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This STON1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-STON1 Antibody

Binding Specificity: AA 660-740

Purpose: Stonin-1 Polyclonal Antibody

Specificity: Stonin-1 Polyclonal Antibody detects endogenous levels of Stonin-1 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the C-terminal region of human Stonin-1 at AA range: 660-740

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:10000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for STON1

Target: STON1 (Stonin 1 (STON1))

Alternative Name: Stonin-1

Background: Rabbit Anti-Stonin-1 Polyclonal Antibody,STON1, SALF, SBLF, STN1, Stonin-1, Stoned B-like factor,Endocytosis of cell surface proteins is mediated by a complex molecular machinery that assembles on the inner surface of the plasma membrane. STON1 encodes one of two human homologs of the Drosophila melanogaster stoned B protein. Stonin-1 is related to components of the endocytic machinery and exhibits a modular structure consisting of an N-terminal proline-rich domain, a central region of homology specific to the human stoned B-like proteins, and a C-terminal region homologous to the mu subunits of adaptor protein (AP) complexes. Read-through transcription of this gene into the neighboring downstream gene, which encodes TFIIA-alpha/beta-like factor, generates a transcript (SALF), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product. Alternative splicing results in multiple transcript variants.,Stonin-1

Molecular Weight: observerd band 83kDa

Gene ID: 11037

UniProt: Q9Y6Q2