SMC1A antibody

Catalog No. ABIN7225230

The Rabbit Polyclonal anti-SMC1A antibody is suitable to detect SMC1A in samples from Human and Mouse. It has been validated for WB, IHC, ELISA and IF.

Quick Overview for SMC1A antibody (ABIN7225230)

Target: SMC1A (Structural Maintenance of Chromosomes 1A (SMC1A))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMC1A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-SMC1A Antibody

Purpose: Rabbit Anti-SMC1 Polyclonal Antibody

Specificity: SMC1 Polyclonal Antibody detects endogenous levels of SMC1 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human SMC1 around the non-phosphorylation site of S957

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:10000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for SMC1A

Target: SMC1A (Structural Maintenance of Chromosomes 1A (SMC1A))

Alternative Name: SMC1

Background: SMC1A, DXS423E, KIAA0178, SB1.8, SMC1, SMC1L1, Structural maintenance of chromosomes protein 1A, SMC protein 1A, SMC-1-alpha, SMC-1A, Sb1.8Proper cohesion of sister chromatids is a prerequisite for the correct segregation of chromosomes during cell division. The cohesin multiprotein complex is required for sister chromatid cohesion. This complex is composed partly of two structural maintenance of chromosomes (SMC) proteins, SMC3 and either SMC1B or the protein encoded by this gene. Most of the cohesin complexes dissociate from the chromosomes before mitosis, although those complexes at the kinetochore remain. Therefore, the encoded protein is thought to be an important part of functional kinetochores. In addition, this protein interacts with BRCA1 and is phosphorylated by ATM, indicating a potential role for this protein in DNA repair. SMC1A (structural maintenance of chromosomes 1A), which belongs to the SMC gene family, is located in an area of the X-chromosome that escapes X inactivation. Mutations in SMC1A result in Cornelia de Lange syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms.

Molecular Weight: 143kD

Gene ID: 8243

UniProt: Q14683

Pathways: Stem Cell Maintenance