TBC1D4 antibody

Catalog No. ABIN7226136

This Rabbit Polyclonal antibody specifically detects TBC1D4 in ELISA, IF and IHC (p). It exhibits reactivity toward Human and Mouse.

Quick Overview for TBC1D4 antibody (ABIN7226136)

Target: TBC1D4 (TBC1 Domain Family, Member 4 (TBC1D4))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TBC1D4 antibody is un-conjugated

Application: ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-TBC1D4 Antibody

Purpose: TBC1D4 Polyclonal Antibody

Specificity: TBC1D4 Polyclonal Antibody detects endogenous levels of TBC1D4 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human TBC1D4 around the non-phosphorylation site of T642

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:10000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for TBC1D4

Target: TBC1D4 (TBC1 Domain Family, Member 4 (TBC1D4))

Alternative Name: TBC1D4

Background: Rabbit Anti-TBC1D4 Polyclonal Antibody,TBC1D4, AS160, KIAA0603, TBC1 domain family member 4, Akt substrate of 160 kDa, AS160,TBC1D4 (TBC1 domain family member 4) is a member of the Tre-2/BUB2/CDC16 domain family. The protein encoded by TBC1D4 is a Rab-GTPase-activating protein, and contains two phopshotyrosine-binding domains (PTB1 and PTB2), a calmodulin-binding domain (CBD), a Rab-GTPase domain, and multiple AKT phosphomotifs. This protein is thought to play an important role in glucose homeostasis by regulating the insulin-dependent trafficking of the glucose transporter 4 (GLUT4), important for removing glucose from the bloodstream into skeletal muscle and fat tissues. Reduced expression of this gene results in an increase in GLUT4 levels at the plasma membrane, suggesting that this protein is important in intracellular retention of GLUT4 under basal conditions. When exposed to insulin, this protein is phosphorylated, dissociates from GLUT4 vesicles, resulting in increased GLUT4 at the cell surface, and enhanced glucose transport. Phosphorylation of this protein by AKT is required for proper translocation of GLUT4 to the cell surface. Individuals homozygous for a mutation in this gene are at higher risk for type 2 diabetes and have higher levels of circulating glucose and insulin levels after glucose ingestion. Alternative splicing results in multiple transcript variants encoding different isoforms.,TBC1 domain family member 4

Gene ID: 9882

UniProt: O60343