TFIIA-alpha (AA 250-330) antibody

Catalog No. ABIN7221386

The Rabbit Polyclonal anti-TFIIA-alpha antibody (ABIN7221386) specifically detects TFIIA-alpha in ELISA, WB and IHC (p). The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for TFIIA-alpha (AA 250-330) antibody (ABIN7221386)

Target: TFIIA-alpha

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details

Binding Specificity: AA 250-330

Purpose: TFIIA-α Polyclonal Antibody

Specificity: TFIIA-α Polyclonal Antibody detects endogenous levels of TFIIA-α protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the C-terminal region of human TFIIA-alpha at AA range: 250-330

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:40000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details

Target: TFIIA-alpha

Background: Rabbit Anti-TFIIA-α Polyclonal Antibody,GTF2A1, TF2A1, Transcription initiation factor IIA subunit 1, General transcription factor IIA subunit 1, TFIIAL, Transcription initiation factor TFIIA 42 kDa subunit, TFIIA-42,Accurate transcription initiation on TATA-containing class II genes involves the ordered assembly of RNA polymerase II (POLR2A, MIM 180660) and several general initiation factors (summarized by DeJong and Roeder, 1993 [PubMed 8224848]). One of these factors is TFIIA, which when purified from HeLa extracts consists of 35-, 19-, and 12-kD subunits.,Transcription initiation factor IIA subunit 1

Molecular Weight: observerd band 42kDa

Gene ID: 2957

UniProt: P52655