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TLE1/2/3/4 (AA 721-770) antibody

The Rabbit Polyclonal anti-TLE1/2/3/4 antibody has been validated for WB and ELISA. It is suitable to detect TLE1/2/3/4 in samples from Human, Mouse and Rat.
Catalog No. ABIN7226814
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$322.23
$379.10
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Quick Overview for TLE1/2/3/4 (AA 721-770) antibody (ABIN7226814)

Target

TLE1/2/3/4

Reactivity

  • 9
  • 9
  • 9
  • 1
Human, Mouse, Rat

Host

  • 9
Rabbit

Clonality

  • 9
Polyclonal

Conjugate

  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Un-conjugated

Application

Western Blotting (WB), ELISA
  • Binding Specificity

    • 1
    • 1
    AA 721-770

    Purpose

    TLE1/2/3/4 Polyclonal Antibody

    Specificity

    TLE1/2/3/4 Polyclonal Antibody detects endogenous levels of TLE1/2/3/4.

    Purification

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

    Immunogen

    Synthesized peptide derived from TLE1/2/3/4 at AA range: 721-770

    Isotype

    IgG
  • Application Notes

    Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:10000-1:20000).

    Comment

    Primary Antibody

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1 mg/mL

    Buffer

    PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    -20 °C

    Storage Comment

    Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
  • Target

    TLE1/2/3/4

    Background

    Rabbit Anti-TLE1/2/3/4 Polyclonal Antibody,similar to transducin-like enhancer of split 1/2/3/4,Transducin-like enhancer of split proteins (TLE1, TLE2, TLE3, TLE4, and TLE6) are mammalian homologs of Drosophila Groucho. TLEs contain several WD-repeats implicated in protein-protein interaction. TLEs are transcriptional co-repressors that bind to many transcription factors such as LEF1, Runx1, Oct-1, hepatocyte nuclear factor 3-β as well as histone H3. TLEs are differentially expressed during animal development and may have overlapping as well as distinct functions.,TLE1

    Molecular Weight

    observerd band 90kDa

    Gene ID

    7088
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