Topo IIalpha (pThr1343) antibody

Catalog No. ABIN7224742

The Rabbit Polyclonal anti-Topo IIalpha antibody is suitable to detect Topo IIalpha in samples from Human. It has been validated for WB and ELISA.

Quick Overview for Topo IIalpha (pThr1343) antibody (ABIN7224742)

Target: Topo IIalpha

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Product Details

Binding Specificity: pThr1343

Purpose: Topo IIα (phospho Thr1343) Polyclonal Antibody

Specificity: Phospho-Topo IIα (T1343) Polyclonal Antibody detects endogenous levels of Topo IIα protein only when phosphorylated at T1343.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human Topo IIalpha Phospho-Thr1343

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:5000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details

Target: Topo IIalpha

Background: Rabbit Anti-Topo IIα (phospho Thr1343) Polyclonal Antibody,TOP2A, TOP2, DNA topoisomerase 2-alpha, DNA topoisomerase II, alpha isozyme,TOP2A (topoisomerase (DNA) II alpha) encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromosome 17 and the beta gene is localized to chromosome 3. TOP2A encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia.,DNA topoisomerase 2-alpha

Molecular Weight: observerd band 190kDa

Gene ID: 7153

UniProt: P11388