TOP2B antibody (AA 10-90)

Catalog No. ABIN7224754

This Rabbit Polyclonal antibody specifically detects TOP2B in WB, ELISA and IHC (p). It exhibits reactivity toward Human and Mouse.

Quick Overview for TOP2B antibody (AA 10-90) (ABIN7224754)

Target: TOP2B (Topoisomerase (DNA) II beta 180kDa (TOP2B))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TOP2B antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-TOP2B Antibody

Binding Specificity: AA 10-90

Purpose: Topo IIβ Polyclonal Antibody

Specificity: Topo IIβ Polyclonal Antibody detects endogenous levels of Topo IIβ protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the N-terminal region of human Topo IIbeta at AA range: 10-90

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:20000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for TOP2B

Target: TOP2B (Topoisomerase (DNA) II beta 180kDa (TOP2B))

Alternative Name: Topo IIbeta

Background: Rabbit Anti-Topo IIβ Polyclonal Antibody,TOP2B, DNA topoisomerase 2-beta, DNA topoisomerase II, beta isozyme,TOP2B (topoisomerase (DNA) II beta) encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, beta, is localized to chromosome 3 and the alpha form is localized to chromosome 17. TOP2B encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in TOP2B have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. Alternative splicing of TOP2B results in two transcript variants, however, the second variant has not yet been fully described.,DNA topoisomerase 2-beta

Molecular Weight: observerd band 183kDa

Gene ID: 7155

UniProt: Q02880