Tropomyosin antibody (N-Term)

Catalog No. ABIN7217388

This Rabbit Polyclonal antibody specifically detects Tropomyosin in WB and ELISA. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for Tropomyosin antibody (N-Term) (ABIN7217388)

Target: Tropomyosin (TPM1) (Tropomyosin 1 (Alpha) (TPM1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Tropomyosin antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Tropomyosin Antibody

Binding Specificity: N-Term

Purpose: Tropomyosin α Polyclonal Antibody

Specificity: Tropomyosin α Polyclonal Antibody detects endogenous levels of Tropomyosin α protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the N-terminal region of human Tropomyosin alpha

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:10000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for Tropomyosin

Target: Tropomyosin (TPM1) (Tropomyosin 1 (Alpha) (TPM1))

Alternative Name: Tropomyosin alpha

Background: Rabbit Anti-Tropomyosin α Polyclonal Antibody,TPM1, C15orf13, TMSA, Tropomyosin alpha-1 chain, Alpha-tropomyosin, Tropomyosin-1,TPM1 is a member of the tropomyosin family of highly conserved, widely distributed actin-binding proteins involved in the contractile system of striated and smooth muscles and the cytoskeleton of non-muscle cells. Tropomyosin is composed of two alpha-helical chains arranged as a coiled-coil. It is polymerized end to end along the two grooves of actin filaments and provides stability to the filaments. The tropomyosin alpha-1 chain is one type of alpha helical chain that forms the predominant tropomyosin of striated muscle, where it also functions in association with the troponin complex to regulate the calcium-dependent interaction of actin and myosin during muscle contraction. In smooth muscle and non-muscle cells, alternatively spliced transcript variants encoding a range of isoforms have been described. Mutations in this gene are associated with type 3 familial hypertrophic cardiomyopathy.,Tropomyosin alpha-1 chain

Molecular Weight: observerd band 35kDa

Gene ID: 7168

UniProt: P09493

Pathways: Regulation of Actin Filament Polymerization