TNNI2 antibody (pSer43)

Catalog No. ABIN7224728

The Rabbit Polyclonal anti-TNNI2 antibody has been validated for WB, ELISA and IHC (p). It is suitable to detect TNNI2 in samples from Human, Mouse and Rat.

Quick Overview for TNNI2 antibody (pSer43) (ABIN7224728)

Target: TNNI2 (Fast Skeletal Troponin I (TNNI2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TNNI2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-TNNI2 Antibody

Binding Specificity: pSer43

Purpose: Troponin I-C (phospho Ser43) Polyclonal Antibody

Specificity: Phospho-Troponin I-C (S43) Polyclonal Antibody detects endogenous levels of Troponin I-C protein only when phosphorylated at S43.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human Troponin I-C Phospho-Ser43

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:40000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for TNNI2

Target: TNNI2 (Fast Skeletal Troponin I (TNNI2))

Alternative Name: Troponin I-C

Background: Rabbit Anti-Troponin I-C (phospho Ser43) Polyclonal Antibody,TNNI3, TNNC1, Troponin I, cardiac muscle, Cardiac troponin I,Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit, blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: tnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. NNI3 (troponin I3, cardiac type) encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in TNNI3 cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM).,Troponin I cardiac muscle

Molecular Weight: observerd band 26kDa

Gene ID: 7137

UniProt: P19429