Ubiquitin antibody

Catalog No. ABIN7212222

The Mouse Monoclonal anti-Ubiquitin antibody has been validated for WB, IF and IHC (p). It is suitable to detect Ubiquitin in samples from Human, Rat and Mouse.

Quick Overview for Ubiquitin antibody (ABIN7212222)

Target: Ubiquitin (Ub)

Reactivity: Human, Rat, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Ubiquitin antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 5F1

Product Details anti-Ubiquitin Antibody

Purpose: Ubiquitin Mouse Monoclonal Antibody (5F1)

Specificity: Ubiquitin protein detects endogenous levels of Ubiquitin.

Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen

Immunogen: Synthetic Peptide of Ubiquitin

Isotype: IgG1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:1000-1:2000), IHC-P (1:100-1:200).

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for Ubiquitin

Target: Ubiquitin (Ub)

Alternative Name: Ubiquitin

Background: Mouse Anti-Ubiquitin Mouse Monoclonal Antibody (5F1),FLJ25987, MGC8385, Polyubiquitin B, RPS 27A, RPS27A, UBA 52, UBA 80, UBA52, UBA80, UBB, UBB_HUMAN, UBC, UBCEP 1, UBCEP 2, UBCEP1, UBCEP2, Ubiquitin, Ubiquitin B,UBB (ubiquitin B) encodes ubiquitin, one of the most conserved proteins known. Ubiquitin has a major role in targeting cellular proteins for degradation by the 26S proteosome. It is also involved in the maintenance of chromatin structure, the regulation of gene expression, and the stress response. Ubiquitin is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin moiety fused to an unrelated protein. UBB consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. An aberrant form of this protein has been detected in patients with Alzheimer's disease and Down syndrome. Pseudogenes of UBB are located on chromosomes 1, 2, 13, and 17. Alternative splicing results in multiple transcript variants.,Ubiquitin

Gene ID: 7314

Pathways: Mitotic G1-G1/S Phases, Ubiquitin Proteasome Pathway