UBD antibody (Internal Region)

Catalog No. ABIN7226618

The Rabbit Polyclonal anti-UBD antibody (ABIN7226618) specifically detects UBD in WB and ELISA. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for UBD antibody (Internal Region) (ABIN7226618)

Target: UBD (Ubiquitin D (UBD))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBD antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-UBD Antibody

Binding Specificity: Internal Region

Purpose: Rabbit Anti-FAT10 Polyclonal Antibody

Specificity: FAT10 Polyclonal Antibody detects endogenous levels of FAT10 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human FAT10

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,ELISA 1:10000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for UBD

Target: UBD (Ubiquitin D (UBD))

Alternative Name: FAT10

Background: UBD, FAT10, Ubiquitin D, Diubiquitin, Ubiquitin-like protein FAT10FAT10, also designated Ubiquitin D or Diubiquitin, is a 165 amino acid protein encoded in the major histocompatibility complex (MHC) that consists of two domains which share significant homology with ubiquitin. Each domain contains two cysteines, along with a free C-terminal diglycine motif required for FAT10 conjugate formation. FAT10 is inducible by interferon-g and tumor necrosis factor a (TNF). The FAT10 protein interacts with MAD2, a component of the spindle checkpoint, and plays a role in antigen presentation, cytokine response, apoptosis and mitosis. It may also regulate cell growth during dendritic cell or B cell activation and development. FAT10 mRNA is expressed mainly in some dendritic cells and lymphoblastoid lines and in other specific cells subsequent to interferon-g induction. The human FAT10 gene, designated UBD, maps to chromosome 6p21. and is overexpressed in the tumors of various epithelial cancers.

Molecular Weight: 18kD

UniProt: O15205

Pathways: Ubiquitin Proteasome Pathway