UBE2O antibody

Catalog No. ABIN7232000

This Rabbit Polyclonal antibody specifically detects UBE2O in WB and ELISA. It exhibits reactivity toward Human and Mouse.

Quick Overview for UBE2O antibody (ABIN7232000)

Target: UBE2O (Ubiquitin-Conjugating Enzyme E2O (UBE2O))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBE2O antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-UBE2O Antibody

Purpose: UBE2O Polyclonal Antibody

Specificity: The antibody detects endogenous levels of UBE2O protein

Purification: The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen

Immunogen: Synthesized peptide derived from part region of human UBE2O protein

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:5000-1:20000).

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium Azide as preservative and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for UBE2O

Target: UBE2O (Ubiquitin-Conjugating Enzyme E2O (UBE2O))

Alternative Name: UBE2O

Background: Rabbit Anti-UBE2O Polyclonal Antibody,(E3-independent) E2 ubiquitin-conjugating enzyme, E2/E3 hybrid ubiquitin-protein ligase UBE2O, Ubiquitin carrier protein O, Ubiquitin-conjugating enzyme E2 O, Ubiquitin-conjugating enzyme E2 of 230 kDa, Ubiquitin-conjugating enzyme E2-230K, Ubiquitin-protein ligase O,E2/E3 hybrid ubiquitin-protein ligase that displays both E2 and E3 ligase activities and mediates monoubiquitination of target proteins. Negatively regulates TRAF6-mediated NF-kappa-B activation independently of its E2 activity. Acts as a positive regulator of BMP7 signaling by mediating monoubiquitination of SMAD6, thereby regulating adipogenesis. Mediates monoubiquitination at different sites of the nuclear localization signal (NLS) of BAP1, leading to cytoplasmic retention of BAP1. Also able to monoubiquitinate the NLS of other chromatin-associated proteins, such as INO80 and CXXC1, affecting their subcellular location. Acts as a regulator of retrograde transport by assisting the TRIM27: mAGEL2 E3 ubiquitin ligase complex to mediate Lys-63-linked ubiquitination of WASHC1, leading to promote endosomal F-actin assembly.,UBE2O

Molecular Weight: observerd band 142kDa

Gene ID: 63893

UniProt: Q9C0C9