UBE4A antibody (N-Term)

Catalog No. ABIN7217712

This anti-UBE4A antibody is a Rabbit Polyclonal antibody detecting UBE4A in WB, ELISA and IHC (p). Suitable for Human, Mouse and Rat.

Quick Overview for UBE4A antibody (N-Term) (ABIN7217712)

Target: UBE4A (Ubiquitination Factor E4A (UBE4A))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBE4A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-UBE4A Antibody

Binding Specificity: N-Term

Purpose: UBE4A Polyclonal Antibody

Specificity: UBE4A Polyclonal Antibody detects endogenous levels of UBE4A protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the N-terminal region of human UBE4A

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:10000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for UBE4A

Target: UBE4A (Ubiquitination Factor E4A (UBE4A))

Alternative Name: UBE4A

Background: Rabbit Anti-UBE4A Polyclonal Antibody,UBE4A, KIAA0126, Ubiquitin conjugation factor E4 A,UBE4A encodes a member of the U-box ubiquitin ligase family. The ubiquitination factor E4A is involved in multiubiquitin chain assembly and plays a critical role in chromosome condensation and separation through the polyubiquitination of securin. Autoantibodies against the encoded protein may be markers for scleroderma and Crohn's disease. A pseudogene of this gene is located on the long arm of chromosome 3. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.,Ubiquitin conjugation factor E4 A

Molecular Weight: observerd band 122kDa

Gene ID: 9354

UniProt: Q14139