CrkL antibody (pTyr207)

Catalog No. ABIN7213320

This Rabbit Polyclonal antibody specifically detects CrkL in WB, ELISA, IF and IHC (p). It exhibits reactivity toward Human, Mouse, Rat and Monkey.

Quick Overview for CrkL antibody (pTyr207) (ABIN7213320)

Target: CrkL (CRKL) (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL))

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CrkL antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CrkL Antibody

Binding Specificity: pTyr207

Purpose: Crk-L (phospho Tyr207) Polyclonal Antibody

Specificity: Phospho-Crk-L (Y207) Polyclonal Antibody detects endogenous levels of Crk-L protein only when phosphorylated at Y207.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human Crk-L Phospho-Tyr207

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:5000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for CrkL

Target: CrkL (CRKL) (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL))

Alternative Name: Crk-L

Background: Rabbit Anti-Crk-L (phospho Tyr207) Polyclonal Antibody,CRKL, Crk-like protein,CRKL encodes a protein kinase (CRK like proto-oncogene, adaptor protein) containing SH2 and SH3 (src homology) domains which has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase, plays a role in fibroblast transformation by BCR-ABL, and may be oncogenic.,Crk-like protein

Molecular Weight: observerd band 39kDa

Gene ID: 1399

UniProt: P46109

Pathways: Interferon-gamma Pathway, Neurotrophin Signaling Pathway, Signaling of Hepatocyte Growth Factor Receptor