VRK3 antibody (Internal Region)

Catalog No. ABIN7222988

The Rabbit Polyclonal anti-VRK3 antibody has been validated for WB and ELISA. It is suitable to detect VRK3 in samples from Human.

Quick Overview for VRK3 antibody (Internal Region) (ABIN7222988)

Target: VRK3 (Vaccinia Related Kinase 3 (VRK3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VRK3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-VRK3 Antibody

Binding Specificity: Internal Region

Purpose: VRK3 Polyclonal Antibody

Specificity: VRK3 Polyclonal Antibody detects endogenous levels of VRK3 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the Internal region of human VRK3

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:40000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for VRK3

Target: VRK3 (Vaccinia Related Kinase 3 (VRK3))

Alternative Name: VRK3

Background: Rabbit Anti-VRK3 Polyclonal Antibody,VRK3, Inactive serine/threonine-protein kinase VRK3, Serine/threonine-protein pseudokinase VRK3, Vaccinia-related kinase 3,VRK3 (vaccinia related kinase 3) encodes a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. In both human and mouse, VRK3 has substitutions at several residues within the ATP binding motifs that in other kinases have been shown to be required for catalysis. In vitro assays indicate the protein lacks phosphorylation activity. The protein, however, likely retains its substrate binding capability. VRK3 is widely expressed in human tissues and its protein localizes to the nucleus. Alternative splicing results in multiple transcripts encoding different isoforms.,Inactive serine threonine-protein kinase VRK3

Molecular Weight: observerd band 53kDa

Gene ID: 51231

UniProt: Q8IV63