GLI1 antibody (AA 460-490)

Catalog No. ABIN7228664

This Rabbit Polyclonal antibody specifically detects GLI1 in WB and ELISA. It exhibits reactivity toward Human and Mouse.

Quick Overview for GLI1 antibody (AA 460-490) (ABIN7228664)

Target: GLI1 (Zinc Finger Protein GLI1 (GLI1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GLI1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-GLI1 Antibody

Binding Specificity: AA 460-490

Purpose: GLI-1 Polyclonal Antibody

Specificity: This antibody detects endogenous levels of GLI-1

Purification: The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen

Immunogen: Synthesized peptide derived from part region of human GLI-1 protein at AA range: 460-490

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:10000-1:20000).

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium Azide as preservative and 50 % Glycerol as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for GLI1

Target: GLI1 (Zinc Finger Protein GLI1 (GLI1))

Alternative Name: GLI-1

Background: Rabbit Anti-GLI-1 Polyclonal Antibody,Zinc finger protein GLI1, Glioma-associated oncogene, Oncogene GLI,GLI1 encodes a member of the Kruppel family of zinc finger proteins. The encoded transcription factor is activated by the sonic hedgehog signal transduction cascade and regulates stem cell proliferation. The activity and nuclear localization of this protein is negatively regulated by p53 in an inhibitory loop. Multiple transcript variants encoding different isoforms have been found for this gene.,GLI-1

Molecular Weight: observerd band 120kDa

Gene ID: 2735

UniProt: P08151

Pathways: Hedgehog Signaling, Dopaminergic Neurogenesis