BNIP3 antibody (C-Term)

Catalog No. ABIN7232776

This Rabbit Polyclonal antibody specifically detects BNIP3 in WB and IF/ICC. It exhibits reactivity toward Human.

Quick Overview for BNIP3 antibody (C-Term) (ABIN7232776)

Target: BNIP3 (BCL2/adenovirus E1B 19kDa Interacting Protein 3 (BNIP3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BNIP3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (fixed cells) (IF/ICC)

Product Details anti-BNIP3 Antibody

Binding Specificity: C-Term

Purpose: Anti-BNIP3 Rabbit Polyclonal Antibody

Specificity: This antibody recognizes human and mouse BNIP3.

Cross-Reactivity: Human, Mouse

Immunogen: Synthetic peptide corresponding to amino acids at the C-terminus of human BNIP3.

Application Details

Application Notes: Immunoblotting: use at 1 μg/mL. A band of ~30 kDa is detected, an additional ~60 kDa band, complexed BNIP3, may also be detected.
Immunofluorescence: use at 10 μg/mL.
These are recommended concentrations.
Endusers should determine optimal concentrations for their applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Dilute in PBS or medium which is identical to that used in the assay system.

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, 50 % glycerol, 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: This antibody is stable for at least one (1) year at -20°C.

Target Details for BNIP3

Target: BNIP3 (BCL2/adenovirus E1B 19kDa Interacting Protein 3 (BNIP3))

Alternative Name: BNIP3

Background: BCL2/adenovirus E1B 19 kDa protein-interacting protein 3,Apoptosis-inducing protein that can overcome BCL2 suppression. May play a role in repartitioning calcium between the two major intracellular calcium stores in association with BCL2. Involved in mitochondrial quality control via its interaction with SPATA18/MIEAP: in response to mitochondrial damage, participates in mitochondrial protein catabolic process (also named MALM) leading to the degradation of damaged proteins inside mitochondria. The physical interaction of SPATA18/MIEAP, BNIP3 and BNIP3L/NIX at the mitochondrial outer membrane regulates the opening of a pore in the mitochondrial double membrane in order to mediate the translocation of lysosomal proteins from the cytoplasm to the mitochondrial matrix. Plays an important role in the calprotectin (S100A8/A9)-induced cell death pathway. {PubMed:19935772, PubMed:22292033}.,BNIP3 is a member of the apoptotic Bcl-2 protein family that is involved in both necrosis and apoptosis. Humans and other animals, as well as lower eukaryotes, encode several BNIP3 paralogues including human BNIP3L which induces apoptosis by interacting with viral and cellular anti-apoptosis proteins. BNIP3 interacts with the E1B 19 kDa protein, which is responsible for the protection of virally induced cell death, as well as with E1B-like sequences of BCL2 which is also an apoptotic protector. The BNIP3 gene contains a BH3 domain and a transmembrane domain that have been associated with pro-apoptotic function. The dimeric mitochondrial protein encoded by the BNIP3 gene is known to induce apoptosis, even in the presence of BCL2.,Mitochondrion. Mitochondrion outer membrane, Single-pass membrane protein. Note=Coexpression with the EIB 19- kDa protein results in a shift in NIP3 localization pattern to the nuclear envelope. Colocalizes with ACAA2 in the mitochondria. Colocalizes with SPATA18 at the mitochondrion outer membrane.

Gene ID: 664

NCBI Accession: NP_004043

UniProt: Q12983

Pathways: Autophagy, Brown Fat Cell Differentiation