CENPA antibody

Catalog No. ABIN7232821

This Mouse Monoclonal antibody specifically detects CENPA in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for CENPA antibody (ABIN7232821)

Target: CENPA (Centromere Protein A (CENPA))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CENPA antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Clone: 5A7-2E11

Product Details anti-CENPA Antibody

Purpose: Anti-CENP-A Mouse Monoclonal Antibody

Specificity: This antibody recognizes human CENP-A. Reactivity with other species has not been investigated.

Cross-Reactivity: Human

Immunogen: Synthetic peptide corresponding to a sequence within human CENP-A.

Isotype: IgG1

Application Details

Application Notes: Immunoblotting: use at 1 μg/mL. A band of ~18 kDa is detected.Detection of CENP-A in lysate of U2OS cellsELISA: use at 1-10 μg/mL with CENP-A on the solid phase.
Positive control: U2OS cell lysate
These are recommended concentrations,
Enduser should determine optimal concentrations for their applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Dilute in PBS or medium that is identical to that used in the assay system.

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, 50 % glycerol, 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: This antibody is stable for at least one (1) year at -20°C.

Target Details for CENPA

Target: CENPA (Centromere Protein A (CENPA))

Alternative Name: CENPA

Background: Histone H3-like centromeric protein A,Histone H3-like nucleosomal protein that is specifically found in centromeric nucleosomes (PubMed:7962047, PubMed:9024683, PubMed:11756469, PubMed:14667408, PubMed:15702419, PubMed:15475964, PubMed:15282608, PubMed:17651496, PubMed:19114591, PubMed:27499292, PubMed:20739937). Replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore (PubMed:18072184). The presence of CENPA subtly modifies the nucleosome structure and the way DNA is wrapped around the nucleosome and gives rise to protruding DNA ends that are less well-ordered and rigid compared to nucleosomes containing histone H3 (PubMed:27499292, PubMed:26878239). May serve as an epigenetic mark that propagates centromere identity through replication and cell division (PubMed:15475964, PubMed:15282608, PubMed:26878239, PubMed:20739937, PubMed:21478274). Required for recruitment and assembly of kinetochore proteins, and as a consequence required for progress through mitosis, chromosome segregation and cytokinesis (PubMed:11756469, PubMed:14667408, PubMed:18072184, PubMed:23818633, PubMed:25556658, PubMed:27499292). {PubMed:11756469, PubMed:14667408, PubMed:15282608, PubMed:15475964, PubMed:15702419, PubMed:17651496, PubMed:18072184, PubMed:19114591, PubMed:21478274, PubMed:23818633, PubMed:25556658, PubMed:26878239, PubMed:27499292, PubMed:7962047, PubMed:9024683, PubMed:20739937}.,Replicated chromosomes include two kinetochores that control chromosome segregation during mitosis. Centromere Protein-A (CENP-A), a histone H3-like protein, contains a C-terminal H3-like domain which is required for centromere localization of CENP-A. It is essential for kinetochore targeting of CENP-C. In the presence of DNA, CENP-A forms an octameric complex with histones H2A, H2B, and H4. CENP-A specifically localizes to active centromeres and is a component of specialized centromeric nucleosomes on which kinetochores are assembled. CENP-A is essential for nucleosomal packaging of centromeric DNA at interphase and functions as a centromere formation marker on chromosomes.,Nucleus, Chromosome, centromere, kinetochore, Chromosome, centromere,Centromere autoantigen A, Centromere protein A, CENP-A

NCBI Accession: NP_001035891

UniProt: P49450

Pathways: Chromatin Binding, Maintenance of Protein Location