RIPK2 antibody (pTyr474)

Catalog No. ABIN7232880

This anti-RIPK2 antibody is a Rabbit Polyclonal antibody detecting RIPK2 in WB and IHC. Suitable for Human.

Quick Overview for RIPK2 antibody (pTyr474) (ABIN7232880)

Target: RIPK2 (Receptor-Interacting Serine-threonine Kinase 2 (RIPK2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RIPK2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-RIPK2 Antibody

Binding Specificity: pTyr474

Purpose: Anti-RIPK2 Rabbit Polyclonal Antibody

Specificity: Human and mouse RIPK2.

Cross-Reactivity: Human, Mouse

Purification: Whole Antiserum

Immunogen: Phosphopeptide corresponding to the pY474 site of human RIPK2.

Application Details

Application Notes: Immunoblotting: use at a dilution of 1:500. Detection of RIPK2 in MDP-stimulated HEK293 cells (+) vs. non-stimulated HEK293 cells (-)
These are recommended dilutions.
Endusers should determine optimal dilutions for their applications.
Immunohistochemistry: use at a dilution of 1:100. Detection of active RIPK2 in human (top) and mouse (bottom) tissues. Non-IBD refers to colon sections from non-inflammatory bowel disease (IBD) patients, IBD refers to colon sections from ulcerative colitis (UC) patients. Mouse strain used: B6.129P2-I110tm1Cgn/J, a common model for IBD that presents with spontaneous colitis that is elevated by DSS (dextran sulfate sodium) exposure.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Dilute in PBS or medium that is identical to that used in the assay system.

Concentration: Lot specific

Buffer: Whole antiserum

Storage: -20 °C

Storage Comment: Store at -20°C in appropriate aliquots to avoid multiple freeze-thaw cycles.

Target Details for RIPK2

Target: RIPK2 (Receptor-Interacting Serine-threonine Kinase 2 (RIPK2))

Alternative Name: RIPK2

Background: Receptor-interacting serine/threonine-protein kinase 2,Serine/threonine/tyrosine kinase that plays an essential role in modulation of innate and adaptive immune responses. Upon stimulation by bacterial peptidoglycans, NOD1 and NOD2 are activated, oligomerize and recruit RIPK2 through CARD-CARD domains. Contributes to the tyrosine phosphorylation of the guanine exchange factor ARHGEF2 through Src tyrosine kinase leading to NF-kappaB activation by NOD2. Once recruited, RIPK2 autophosphorylates and undergoes 'Lys-63'-linked polyubiquitination by E3 ubiquitin ligases XIAP, BIRC2 and BIRC3. The polyubiquitinated protein mediates the recruitment of MAP3K7/TAK1 to IKBKG/NEMO and induces 'Lys-63'-linked polyubiquitination of IKBKG/NEMO and subsequent activation of IKBKB/IKKB. In turn, NF-kappa-B is released from NF-kappa-B inhibitors and translocates into the nucleus where it activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. Plays also a role during engagement of the T-cell receptor (TCR) in promoting BCL10 phosphorylation and subsequent NF-kappa-B activation. Plays a role in the inactivation of RHOA in response to NGFR signaling (PubMed:26646181). {PubMed:14638696, PubMed:17054981, PubMed:18079694, PubMed:21123652, PubMed:21887730, PubMed:26646181}.,Cytoplasm

Gene ID: 603455

UniProt: O43353

Pathways: TCR Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Toll-Like Receptors Cascades