ATG12 antibody (C-Term)

Catalog No. ABIN7232888

The Rabbit Polyclonal anti-ATG12 antibody has been validated for WB and IF/ICC. It is suitable to detect ATG12 in samples from Human.

Quick Overview for ATG12 antibody (C-Term) (ABIN7232888)

Target: ATG12 (Autophagy Related 12 (ATG12))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATG12 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (fixed cells) (IF/ICC)

Product Details anti-ATG12 Antibody

Binding Specificity: C-Term

Purpose: Anti-Atg12 Rabbit Polyclonal Antibody

Specificity: This antibody recognizes human Atg12.

Cross-Reactivity: Human

Immunogen: Synthetic peptide corresponding to amino acids at the C-terminus of human Atg12.

Application Details

Application Notes: Immunoblotting: use at 1-2 μg/mL. Bands of ~55 kDa, corresponding to Atg12-Atg5 complex, and ~15kda, corresponding to free Atg12, are detected.
Immunofluorescence: use at 10 μg/mL.
These are recommended concentrations.
Endusers should determine optimal concentrations for their applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Dilute in PBS or medium that is identical to that used in the assay system.

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, 50 % glycerol, 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: This antibody is stable for at least one (1) year at -20°C.

Target Details for ATG12

Target: ATG12 (Autophagy Related 12 (ATG12))

Alternative Name: ATG12

Background: Ubiquitin-like protein ATG12,Ubiquitin-like protein involved in autophagy vesicles formation. Conjugation with ATG5 through a ubiquitin-like conjugating system involving also ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. {PubMed:12207896, PubMed:17999726, PubMed:19074260, PubMed:19164948, PubMed:23202584}., (Microbial infection) May act as a proviral factor. In association with ATG5, negatively regulates the innate antiviral immune response by impairing the type I IFN production pathway upon vesicular stomatitis virus (VSV) infection (PubMed:17709747). Required for the translation of incoming hepatitis C virus (HCV) RNA and, thereby, for the initiation of HCV replication, but not required once infection is established (PubMed:19666601). {PubMed:17709747, PubMed:19666601}.,Autophagy is a catabolic process that results in the degradation of bulk cytoplasmic contents within autophagosomes and lysosomes. Atg12 is an ubiquitin-like protein involved in autophagy vesicle formation. Conjugation with Atg5 through a ubiquitin-like conjugating system involving also Atg7 as an E1-like activating enzyme and Atg10 as an E2-like conjugating enzyme, is essential for its function. The Atg12-Atg5 conjugate acts as an E3-like enzyme which is required for lipidation of Atg8 family proteins and their association to vesicle membranes. The Atg12-Atg5 conjugate also negatively regulates the innate antiviral immune response by blocking the type I IFN production pathway through direct association with RARRES3 and MAVS.,Cytoplasm, Preautophagosomal structure membrane,Autophagy-related protein 12, APG12-like

UniProt: O94817

Pathways: Autophagy