CD57 antibody

Catalog No. ABIN7232911

This Mouse Monoclonal antibody specifically detects CD57 in IHC. It exhibits reactivity toward Human.

Quick Overview for CD57 antibody (ABIN7232911)

Target: CD57 (B3GAT1) (beta-1,3-Glucuronyltransferase 1 (Glucuronosyltransferase P) (B3GAT1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD57 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: G539-1

Product Details anti-CD57 Antibody

Purpose: Anti-CD57 Mouse Monoclonal Antibody

Specificity: Human CD57. Reactivity with other species has not been investigated.

Cross-Reactivity: Human

Immunogen: Recombinant human CD57.

Isotype: IgG1

Application Details

Application Notes:
Immunohistochemistry: use at a dilution of 1:100-1:200 on formalin-fixed, paraffin-embedded samples after heat-induced epitope retrieval at pH 9 for 10-30 minutes.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Dilute in PBS or medium that is identical to that used in the assay system.

Concentration: Lot specific

Buffer: Tris buffer, pH 7.3-7.7, 1 % BSA, 0.1 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

Target Details for CD57

Target: CD57 (B3GAT1) (beta-1,3-Glucuronyltransferase 1 (Glucuronosyltransferase P) (B3GAT1))

Alternative Name: B3GAT1

Background: Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1,Involved in the biosynthesis of L2/HNK-1 carbohydrate epitope on glycoproteins. Can also play a role in glycosaminoglycan biosynthesis. Substrates include asialo-orosomucoid (ASOR), asialo-fetuin, and asialo-neural cell adhesion molecule. Requires sphingomyelin for activity: stearoyl-sphingomyelin was the most effective, followed by palmitoyl-sphingomyelin and lignoceroyl-sphingomyelin. Activity was demonstrated only for sphingomyelin with a saturated fatty acid and not for that with an unsaturated fatty acid, regardless of the length of the acyl group. {UniProtKB:O35789}.,It was originally believed that CD57 (cluster of differentiation 57) was uniquely expressed on natural killer (NK) cells, but further research showed that CD57 was expressed only on a subset of functionally distinct NK cells. CD57 was subsequently identified on CD8+ T cells as well as on cells of neural crest origin. In neural cells, the CD57 epitope is predominantly restricted to adhesion molecules. CD57 has been utilized as a marker for tumors of neuroendocrine origin including pheochromocytomas, paragangliomas, carcinoid tumors, medulloblastomas, neuromas, neurofibromas, schwannomas, and granular cell tumors. Expression of CD57 can be used to distinguish nodular lymphocyte-predominant Hodgkin's lymphoma from T-cell/histiocyte-rich large B-cell lymphoma, nodular sclerosis, Hodgkin's disease, and follicular lymphoma.,[Isoform 1]: Golgi apparatus membrane, Secreted, Endoplasmic reticulum membrane, Secreted,EC 2.4.1.135, Beta-1,3-glucuronyltransferase 1, Glucuronosyltransferase P, GlcAT-P, UDP-GlcUA:glycoprotein beta-1,3-glucuronyltransferase, GlcUAT-P

UniProt: Q9P2W7

Pathways: Glycosaminoglycan Metabolic Process