Rho-related GTP-binding protein antibody

Catalog No. ABIN7233029

The Mouse Monoclonal anti-Rho-related GTP-binding protein antibody has been validated for ELISA, WB, IHC, IP and IF/ICC. It is suitable to detect Rho-related GTP-binding protein in samples from Mammalian.

Quick Overview for Rho-related GTP-binding protein antibody (ABIN7233029)

Target: Rho-related GTP-binding protein (RhO (pan))

Reactivity: Mammalian

Host: Mouse

Clonality: Monoclonal

Conjugate: This Rho-related GTP-binding protein antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (fixed cells) (IF/ICC)

Clone: 4D2

Product Details anti-Rho-related GTP-binding protein Antibody

Purpose: Anti-Rhodopsin Mouse Monoclonal Antibody

Specificity: This antibody recognizes the N-terminus of Rhodopsin from all mammalian species tested (human, mouse, rat, bovine, porcine), and most fish, birds, and amphibians.

Cross-Reactivity: Amphibian, Avian, Fish, Mammalian

Immunogen: Bovine rhodopsin

Isotype: IgG1

Application Details

Application Notes: Immunoblotting: use at 1-10 μg/mL. A band of ~40 kDa is detected.
Immunohistochemistry: use at 1-10 μg/mL, paraffin-embedded and frozen.
Immunoprecipitation: use at 1-2 μg per 100-500 μg of protein.
These are recommended concentrations. User should determine optimal concentrations for their application.
Positive control: Rat eye lysate

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Dilute in PBS or medium that is identical to that used in the assay system.

Concentration: Lot specific

Buffer: PBS, pH 7.4, 50 % glycerol, 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: This antibody is stable for at least one (1) year at -20°C.

Target Details for Rho-related GTP-binding protein

Target: Rho-related GTP-binding protein (RhO (pan))

Alternative Name: RHO

Background: Rhodopsin,Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth (By similarity). Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change that activates signaling via G-proteins (PubMed:10926528, PubMed:12044163, PubMed:11972040, PubMed:16908857, PubMed:16586416, PubMed:17060607, PubMed:17449675, PubMed:18818650, PubMed:21389983, PubMed:22198838, PubMed:23579341, PubMed:25205354, PubMed:27458239). Subsequent receptor phosphorylation mediates displacement of the bound G-protein alpha subunit by the arrestin SAG and terminates signaling (PubMed:1396673, PubMed:15111114). {UniProtKB:P08100, PubMed:1396673, PubMed:16586416, PubMed:16908857, PubMed:17060607, PubMed:17449675, PubMed:18818650, PubMed:21389983, PubMed:22198838, PubMed:23579341, PubMed:25205354, PubMed:27458239, PubMed:10926528, PubMed:11972040, PubMed:12044163, PubMed:15111114, PubMed:26526852}.,Rhodopsin is comprised of the protein opsin and a reversibly covalently bound cofactor, retinal. Opsin, made up of seven membrane-embedded alpha helices, binds retinal, a photoreactive chromophore, in a central pocket. In addition to being the pigment of the retina that is responsible for the formation of photoreceptor cells, rhodopsin conveys information stored in the chromophore to the surface of the molecule upon light absorption. Mutations in the rhodopsin gene lead to retinitis pigmentosa.,Membrane, Cell projection, cilium, photoreceptor outer segment

UniProt: P02699