GIT1 antibody (C-Term)

Catalog No. ABIN7233038

The Mouse Monoclonal anti-GIT1 antibody has been validated for WB, IP and IF/ICC. It is suitable to detect GIT1 in samples from Rat.

Quick Overview for GIT1 antibody (C-Term) (ABIN7233038)

Target: GIT1

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This GIT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (fixed cells) (IF/ICC)

Clone: S39B-8

Product Details anti-GIT1 Antibody

Binding Specificity: AA 375-770, C-Term

Purpose: Anti-GIT1 Mouse Monoclonal Antibody

Specificity: This antibody recognizes human, mouse and rat GIT1. It does not cross-react with GIT2.

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein G affinity chromatography.

Immunogen: Fusion protein corresponding to aa 375-770 (C-terminus) of rat GIT1 (accession no.Q9Z272).

Isotype: IgG1

Application Details

Application Notes: Immunoblotting: use at 1 μg/mL. Predicted molecular weight is ~90 kDa.
Positive control: rat brain lysate.
These are recommended concentrations.
Endusers should determine optimal concentrations for their applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Dilute in PBS or medium that is identical to that used in the assay system.

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, 50 % glycerol, 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: This antibody is stable for at least one (1) year at -20°C.

Target Details for GIT1

Target: GIT1

Alternative Name: Git1

Background: ARF GTPase-activating protein GIT1,G-protein coupled receptor (GPCR) kinase interacting proteins 1 and 2 (GIT1 and GIT2) are highly conserved ubiquitous scaffold proteins involved in localized signaling to help regulate focal contact assembly and cytoskeletal dynamics. GIT proteins contain multiple interaction domains for small GTPases, kinases, the Rho family GEF PIX, and the focal adhesion protein paxillin. GIT1 is localized to focal adhesions, cyto- plasmic complexes and membrane protrusions, and it regulates cell protrusion formation and cell migration.

UniProt: Q9Z272