MFN2 antibody (AA 370-600)

Catalog No. ABIN7233053

The Mouse Monoclonal anti-MFN2 antibody has been validated for WB, IHC and IF/ICC. It is suitable to detect MFN2 in samples from Mouse.

Quick Overview for MFN2 antibody (AA 370-600) (ABIN7233053)

Target: MFN2 (Mitofusin 2 (MFN2))

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This MFN2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (fixed cells) (IF/ICC)

Clone: S153-5

Product Details anti-MFN2 Antibody

Binding Specificity: AA 370-600

Purpose: Anti-Mitofusin-2 Mouse Monoclonal Antibody

Specificity: This antibody recognizes human, mouse, and rat Mitofusin-2. It does not cross- react with Mitofusin-1.

Cross-Reactivity: Human, Mouse, Rat

Immunogen: Fusion protein corresponding to aa 370-600 (cytoplasmic N-terminus) of mouse Mitofusin-2. This sequence is 97

Isotype: IgG2a

Application Details

Application Notes: Immunoblotting: use at 1-5 μg/mL. A band of ~90 kDa is detected.
Immunofluorescence: use at 10 μg/mL.
These are recommended concentrations.
Enduser should determine optimal concentrations for their application.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Dilute in PBS or medium that is identical to that used in the assay system.

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, 0.1 % sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: This product is stable for at least one (1) year at -20°C.

Target Details for MFN2

Target: MFN2 (Mitofusin 2 (MFN2))

Alternative Name: Mfn2

Background: Mitofusin-2,Mitochondrial outer membrane GTPase that mediates mitochondrial clustering and fusion (PubMed:12527753, PubMed:23921378, PubMed:23620051). Mitochondria are highly dynamic organelles, and their morphology is determined by the equilibrium between mitochondrial fusion and fission events. Overexpression induces the formation of mitochondrial networks. Membrane clustering requires GTPase activity and may involve a major rearrangement of the coiled coil domains (By similarity). Plays a central role in mitochondrial metabolism and may be associated with obesity and/or apoptosis processes. Plays an important role in the regulation of vascular smooth muscle cell proliferation (By similarity). Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy). Is required for PRKN recruitment to dysfunctional mitochondria (PubMed:23620051). Involved in the control of unfolded protein response (UPR) upon ER stress including activation of apoptosis and autophagy during ER stress (PubMed:23921556). Acts as an upstream regulator of EIF2AK3 and suppresses EIF2AK3 activation under basal conditions (PubMed:23921556). {UniProtKB:O95140, UniProtKB:Q8R500, PubMed:12527753, PubMed:23620051, PubMed:23921378, PubMed:23921556}.,Mitofusin-2 (Mfn2) is a mitochondrial membrane protein that participates in mitochondrial fusion and contributes to the maintenance and operation of the mitochondrial network. This protein is involved in the regulation of vascular smooth muscle cell proliferation, and it may play a role in the pathophysiology of obesity. Mutations in this gene cause Charcot-Marie-Tooth disease type 2A2 and hereditary motor and sensory neuropathy VI, both of which are disorders of the peripheral nervous system. Defects in this gene have also been associated with early-onset stroke. Two transcript variants encoding the same protein have been identified.,Mitochondrion outer membrane,EC 3.6.5.-, Hypertension-related protein 1, Mitochondrial assembly regulatory factor, HSG protein, Transmembrane GTPase MFN2

UniProt: Q80U63

Pathways: Skeletal Muscle Fiber Development