Digoxigenin antibody

Catalog No. ABIN7233195

The Mouse Monoclonal anti-Digoxigenin antibody (Clone 21H8) (ABIN7233195) specifically detects Digoxigenin in ELISA, WB and IHC. The antibody is reactive with Digitalis purpurea (Common foxglove) samples.

Quick Overview for Digoxigenin antibody (ABIN7233195)

Target: Digoxigenin (DIG)

Reactivity: Digitalis purpurea (Common foxglove)

Host: Mouse

Clonality: Monoclonal

Conjugate: This Digoxigenin antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Clone: 21H8

Product Details anti-Digoxigenin Antibody

Purpose: Anti-Digoxigenin Mouse Monoclonal Antibody

Specificity: This antibody reacts with free and bound digoxigenin.

Cross-Reactivity: Digitalis purpurea (Common foxglove)

Immunogen: Digoxigenin conjugate.

Isotype: IgG1

Application Details

Application Notes: ELISA: use at 0.1-1.0 μg/mL
Immunoblotting:
User should use at 2-5 μg/mL.
Immunohistochemistry: Use at 1-5 μg/mL.
These are recommended concentrations.
End user should determine optimal concentrations for their applications.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute lyophilized antibody using ultrapure to a final concentration no less than 100 μg/mL. Reconstitution with PBS or Tris buffer is acceptable if required. Protein carrier, biocide or cryopreservative can be added as needed. Aliquot and store at -20C for longterm storage. Dilute immediately prior to use.

Buffer: 0.1M Tris, 0.1M glycine, 2 % sucrose

Storage: -20 °C,-80 °C

Storage Comment: This product is stable for at least one (1) year at -20°C to -70°C. Reconstituted product should be stored in appropriate aliquots to avoid repeated freeze-thaw cycles.

Target Details for Digoxigenin

Target: Digoxigenin (DIG)

Alternative Name: Digoxigenin

Target Type: Chemical

Background: Digoxigenin (DIG) is a steroid found exclusively in the flowers and leaves of the plants Digitalis purpurea, Digitalis orientalis and Digitalis lanata (foxgloves). It is used in many molecular biology applications similarly to other popular haptens such as DNP (dinitrophenol), biotin, and fluorescein and is often used as an immunohistochemical marker for in situ hybridization. In this case it is conjugated to a single species of RNA nucleotide triphosphate (typically uridine), which is then incorporated into RNA as it is synthesized.

CAS-No: 1672-46-4