TGM3 antibody

Catalog No. ABIN7233203

The Mouse Monoclonal anti-TGM3 antibody has been validated for WB, IHC and ICC. It is suitable to detect TGM3 in samples from Human.

Quick Overview for TGM3 antibody (ABIN7233203)

Target: TGM3 (Transglutaminase 3 (E Polypeptide, Protein-Glutamine-gamma-Glutamyltransferase) (TGM3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TGM3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Clone: C2D

Product Details anti-TGM3 Antibody

Purpose: Anti-Transglutaminase-3 (TG3) Mouse Monoclonal Antibody

Specificity: This antibody recognizes full-length human TG3 (77 kDa) and the proteolysed form of human TG3 (30-47 kDa).

Cross-Reactivity: Human

Immunogen: Recombinant human TG3 (accession no. Q08188).

Isotype: IgG1

Application Details

Application Notes: Immunoblotting: use at 1-5 μg/mL. Bands of ~77 kDa and ~30 kDa are detected.
Immunocytochemistry: use at 2-10 μg/mL.
These are recommended concentrations.
Enduser should determine optimal concentrations for their applications.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute lyophilized antibody using ultrapure to a final concentration no less than 100 μg/mL. Reconstitution with PBS or Tris buffer is acceptable if required. Protein carrier, biocide or cryopreservative can be added as needed. Aliquot and store at -20C for longterm storage. Dilute immediately prior to use.

Buffer: 0.1M Tris, 0.1M glycine, 2 % sucrose

Storage: -20 °C,-80 °C

Storage Comment: This product is stable for at least one (1) year at -20°C to -70°C. Reconstituted product should be stored in appropriate aliquots to avoid repeated freeze-thaw cycles.

Target Details for TGM3

Target: TGM3 (Transglutaminase 3 (E Polypeptide, Protein-Glutamine-gamma-Glutamyltransferase) (TGM3))

Alternative Name: TGM3

Background: Protein-glutamine γ-glutamyltransferase E,Catalyzes the calcium-dependent formation of isopeptide cross-links between glutamine and lysine residues in various proteins, as well as the conjugation of polyamines to proteins. Involved in the formation of the cornified envelope (CE), a specialized component consisting of covalent cross-links of proteins beneath the plasma membrane of terminally differentiated keratinocytes. Catalyzes small proline-rich proteins (SPRR1 and SPRR2) and LOR cross-linking to form small interchain oligomers, which are further cross-linked by TGM1 onto the growing CE scaffold (By similarity). In hair follicles, involved in cross-linking structural proteins to hardening the inner root sheath. {ECO:0000250}.,Transglutaminase-3 (TG3), an epidermal- type enzyme, is responsible for formation of the epidermis. During keratinocyte differentiation, TG1 and TG3 appear to act cooperatively in the cross-linking of proteins, including involucrin, loricrin and small proline-rich proteins (SPRs). These reactions result in formation of the cornified envelope (CE), a specialized component consisting of covalent cross- links of proteins beneath the plasma membrane of terminally differentiated keratinocytes. Furthermore, TG3 in hair follicles is involved in cross-linking structural proteins such as trichohyalin and keratin intermediate to hardening the inner root sheath. During differentiation in these processes, a zymogen form of TG3 (77 kDa) is activated by limited proteolysis with cathepsins S and/or L.,Cytoplasm,EC 2.3.2.13, Transglutaminase E, TG(E, TGE, TGase E, Transglutaminase-3, TGase-3 [Cleaved into: Protein-glutamine γ-glutamyltransferase E 50 kDa catalytic chain, Protein-glutamine γ-glutamyltransferase E 27 kDa non-catalytic chain]

UniProt: Q08188