Acid Phosphatase antibody (AA 71-158) (HRP)

Catalog No. ABIN716975

The Rabbit Polyclonal anti-Acid Phosphatase antibody has been validated for WB, ELISA, IHC (p) and IHC (fro). It is suitable to detect Acid Phosphatase in samples from Mouse.

Quick Overview for Acid Phosphatase antibody (AA 71-158) (HRP) (ABIN716975)

Target: Acid Phosphatase (ACP)

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Acid Phosphatase antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Acid Phosphatase Antibody (HRP)

Binding Specificity: AA 71-158

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Rat,Dog,Cow,Pig,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Acid Phosphatase

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Acid Phosphatase

Target: Acid Phosphatase (ACP)

Alternative Name: Acid Phosphatase

Background:

Synonyms: Acid phosphatase 1 soluble, Acid phosphatase of erythrocyte, Adipocyte acid phosphatase, Cytoplasmic phosphotyrosyl protein phosphatase, HAAP, Low molecular weight phosphotyrosine protein phosphatase, PAP1, PAP2, Protein tyrosine phosphatase, PTPase, Purple acid phosphatase, Red cell acid phosphatase 1, PPAC_HUMAN.

Background: Phosphatase enzymes catalyse hydrolysis of phosphoric acid esters of various alcohols, e.g. a hexose phosphate, to yield the alcohol and free inorganic phosphate. This may be a way of recycling phosphate in the cell, and the level of phosphate may be elevated under conditions of phosphate starvation (in algae for example). Acid phosphatases have pH optima below 7, whereas alkaline phosphatases are most active above pH 7. Commercial quantities of acid phosphatase are obtained from plant sources e.g. potato and wheat germ, whereas similar quantities of alkaline phosphatase are obtained from animal intestine and bacteria.

Gene ID: 5068