G Protein-Coupled Receptor 132 antibody (Extracellular)

Catalog No. ABIN7237639

The Rabbit Polyclonal anti-G Protein-Coupled Receptor 132 antibody has been validated for WB, FACS and LCI. It is suitable to detect G Protein-Coupled Receptor 132 in samples from Mouse.

Quick Overview for G Protein-Coupled Receptor 132 antibody (Extracellular) (ABIN7237639)

Target: G Protein-Coupled Receptor 132 (GPR132)

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This G Protein-Coupled Receptor 132 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Product Details anti-G Protein-Coupled Receptor 132 Antibody

Binding Specificity: AA 184-198, Extracellular

Purpose: A Rabbit Polyclonal antibody to GPR132 (extracellular)

Specificity: Extracellular, 2nd loop.

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Rat - 11 out of 15 amino acid residues identical It won't recognize human GPR132

Characteristics: Extracellular, 2nd loop

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: GST fusion protein,

Immunogen Sequence: Peptide CFEPLRMNSKIAGYH, corresponding to amino acid residues 184 - 198 of mouse GPR132

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: (ABIN7235365)

Blocking Peptide: (ABIN7235365)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for G Protein-Coupled Receptor 132

Target: G Protein-Coupled Receptor 132 (GPR132)

Alternative Name: GPR132 

Background: G Protein-Coupled Receptor 132, G2 Accumulation Protein, G2A,G-protein coupled receptor 132, (GPR132, G2A) is classified as a member of the proton sensing G-protein coupled receptor (GPCR) subfamily. Like other members of this subfamily, i.e. GPR4, OGR1 (GPR68), and TDAG8 (GPR65), G2A is expressed on the cell surface membrane and contains seven transmembrane domains, similar to serpentine receptors. GPR132 senses changes in extracellular pH , and activation of this proton-sensing receptor by acidosis transduces multiple downstream G protein signaling pathways1.GPR132 is expressed in a broad range of immunoregulatory cell types, including macrophages, dendritic cells, neutrophils, mast cells, T and B lymphocytes. It mediates immune cell migration as well as phagocytosis and regulates autoimmunity and lymphocyte homeostasis2.G2A also plays a role in tumor suppression: the tumor microenvironment is acidic due to glycolytic cancer cell metabolism, hypoxia, and deficient blood perfusion and hence, G2A and other proton sensing receptors may be involved in regulation of cancer cell metastasis and proliferation. Small molecule modulators of the pH -sensing GPCRs are being actively developed and evaluated. Research on pH -sensing GPCRs provides important insights into the molecular interaction between the tumor and its acidic microenvironment and may identify new targets for cancer therapy3.

Alternative names: G Protein-Coupled Receptor 132, G2 Accumulation Protein, G2A

Gene ID: 56696

NCBI Accession: NM_013345

UniProt: Q9Z282