GPR139 antibody (Intracellular, Loop 3)

Catalog No. ABIN7886456

This Rabbit Polyclonal antibody specifically detects GPR139 in IHC and WB. It exhibits reactivity toward Mouse.

Quick Overview for GPR139 antibody (Intracellular, Loop 3) (ABIN7886456)

Target: GPR139 (G Protein-Coupled Receptor 139 (GPR139))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPR139 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Product Details anti-GPR139 Antibody

Binding Specificity: AA 204-217, Intracellular, Loop 3

Purpose: A Rabbit Polyclonal antibody to Parkin

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat,Human - identical

Characteristics: Intracellular, 3rd loop

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)RKSNFRLRGYSTGK, corresponding to amino acid residues 204 - 217 of mouse GPR139 

Isotype: IgG

Application Details

Application Notes:

WB: 1:200-1:800

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:600

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7235369)

Blocking Peptide: (ABIN7235369)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for GPR139

Target: GPR139 (G Protein-Coupled Receptor 139 (GPR139))

Alternative Name: GPR139 

Background:

Synonyms: G Protein-Coupled Receptor 139, PGR3, G(Q)-Coupled Orphan Receptor GPRg1, GPRG1

Description: G-protein-coupled receptors (GPCRs), are the largest family of membrane proteins and mediate most of the physiological responses to hormones, neurotransmitters and environmental stimulants. At the most basic level, all GPCRs are characterized by the presence of seven membrane-spanning α-helical segments separated by alternating intracellular and extracellular loop regions1. Orphan GPCRs are a group of GPCRs that their endogenous ligands remain unidentified and therefore their natural functions in doubt2.GPR139 is an orphan GPCRs for which amino acids L-tryptophan and L-phenylalanine, the adrenocorticotropic hormone (ACTH), and α- and β-melanocyte-stimulating hormone have been proposed as endogenous agonists3. GPR139 was found to be highly conserved across species, as the amino acid sequence of the human GPR139 protein is 96 % , 92 % and 70 % identical to the mouse, chicken and zebrafish orthologs, respectively. According to mRNA expression levels GPR139 is mainly expressed in the central nervous system (CNS). GPR139 has been reported to activate several G protein pathways of which Gq/11 is the primary one4.

Gene ID: 209776

NCBI Accession: NM_001002911

UniProt: Q80UC8