GPR142 antibody (Extracellular)

Catalog No. ABIN7237652

The Rabbit Polyclonal anti-GPR142 antibody is suitable to detect GPR142 in samples from Mouse. It has been validated for WB, FACS, LCI and IHC.

Quick Overview for GPR142 antibody (Extracellular) (ABIN7237652)

Target: GPR142 (G Protein-Coupled Receptor 142 (GPR142))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPR142 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Live Cell Imaging (LCI), Immunohistochemistry (IHC)

Product Details anti-GPR142 Antibody

Binding Specificity: AA 291-304, Extracellular

Purpose: A Rabbit Polyclonal antibody to GPR142 (extracellular)

Specificity: Extracellular, 3rd loop.

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat,human - identical

Characteristics: Extracellular, 3rd loop

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)APVHRDWRVHLALD, corresponding to amino acid residues 291 - 304 of mouse GPR142

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:1000

Application Dilutions Western blot wb: 1:400

Comment:

Negative Control: (ABIN7235372)

Blocking Peptide: (ABIN7235372)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for GPR142

Target: GPR142 (G Protein-Coupled Receptor 142 (GPR142))

Alternative Name: GPR142

Background: G-Protein Coupled Receptor 142, Probable G-Protein Coupled Receptor 142, PGR2,G-protein-coupled receptors (GPCRs) are the largest family of membrane proteins and mediate a myriad of physiological and pathophysiological signaling processes. Of these receptors, all are seven membrane spanning receptors but not all are G protein binding but it is convenient to refer to the receptors as GPCRs1.The GPR142 is a GPCR selectively activated by aromatic amino acids and share 33 % amino acid identity with GPR139. L-tryptophan representing one of the most potent ligands, having an EC50 values of 0.2 - 1 mM2. GPR142 is expressed in both rodent and human pancreatic beta cells. Moreover, it was found to be implicated in the regulation of insulin, thereby having a crucial role in Type II diabetes management3. Moreover, GPR142 is highly expressed in immune cells and its ligand L-tryptophan and metabolite serotonin are known to take part in inflammation process. It was shown that mice lacking GPR142 gene are more resilient to anti-type II collagen (CII) antibody-induced arthritis however the exact mechanism is currently unknown4.

Alternative names: G-Protein Coupled Receptor 142, Probable G-Protein Coupled Receptor 142, PGR2

Gene ID: 217302

NCBI Accession: NM_181790

UniProt: Q7TQN9