GPR142 antibody (Extracellular, Loop 3)

Catalog No. ABIN7886464

The Rabbit Polyclonal anti-GPR142 antibody is suitable to detect GPR142 in samples from Mouse. It has been validated for WB, FACS, LCI and IHC.

Quick Overview for GPR142 antibody (Extracellular, Loop 3) (ABIN7886464)

Target: GPR142 (G Protein-Coupled Receptor 142 (GPR142))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPR142 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Live Cell Imaging (LCI), Immunohistochemistry (IHC)

Product Details anti-GPR142 Antibody

Binding Specificity: AA 291-304, Extracellular, Loop 3

Purpose: A Rabbit Polyclonal antibody to GPR142 (extracellular)

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat,human - identical

Characteristics: Extracellular, 3rd loop

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)APVHRDWRVHLALD, corresponding to amino acid residues 291 - 304 of mouse GPR142

Isotype: IgG

Application Details

Application Notes:

WB: 1:400

FC: 2.5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: 1:1000

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7235372)

Blocking Peptide: (ABIN7235372)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for GPR142

Target: GPR142 (G Protein-Coupled Receptor 142 (GPR142))

Alternative Name: GPR142

Background:

Synonyms: G-Protein Coupled Receptor 142, Probable G-Protein Coupled Receptor 142, PGR2

Description: G-protein-coupled receptors (GPCRs) are the largest family of membrane proteins and mediate a myriad of physiological and pathophysiological signaling processes. Of these receptors, all are seven membrane spanning receptors but not all are G protein binding but it is convenient to refer to the receptors as GPCRs1.The GPR142 is a GPCR selectively activated by aromatic amino acids and share 33 % amino acid identity with GPR139. L-tryptophan representing one of the most potent ligands, having an EC50 values of 0.2 - 1 mM2. GPR142 is expressed in both rodent and human pancreatic beta cells. Moreover, it was found to be implicated in the regulation of insulin, thereby having a crucial role in Type II diabetes management3. Moreover, GPR142 is highly expressed in immune cells and its ligand L-tryptophan and metabolite serotonin are known to take part in inflammation process. It was shown that mice lacking GPR142 gene are more resilient to anti-type II collagen (CII) antibody-induced arthritis however the exact mechanism is currently unknown4.

Gene ID: 217302

NCBI Accession: NM_181790

UniProt: Q7TQN9