GPR174 antibody (Extracellular) (FITC)

Catalog No. ABIN7237657

This anti-GPR174 antibody is a Rabbit Polyclonal antibody detecting GPR174 in FACS and LCI. Suitable for Human.

Quick Overview for GPR174 antibody (Extracellular) (FITC) (ABIN7237657)

Target: GPR174 (G Protein-Coupled Receptor 174 (GPR174))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPR174 antibody is conjugated to FITC

Application: Flow Cytometry (FACS), Live Cell Imaging (LCI)

Product Details anti-GPR174 Antibody (FITC)

Binding Specificity: AA 160-175, Extracellular

Purpose: A Rabbit Polyclonal Antibody to GPR174 (extracellular) conjugated to the fluorescent dye FITC

Specificity: 2nd extracellular loop.

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Human - 15,16 amino acid residues identical, mouse,rat - 13

Characteristics: Extracellular, 2nd loop

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)DTSGNRTK(S)FVDLPTR, corresponding to amino acid residues 160 - 175 of human GPR174

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: N/A

Comment:

Negative Control: (ABIN7582044)

Blocking Peptide: (ABIN7235384)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 15 μL or 50 μL double distilled water (DDW), depending on the sample size.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for GPR174

Target: GPR174 (G Protein-Coupled Receptor 174 (GPR174))

Alternative Name: GPR174

Background: G-protein coupled receptor 174, LYPSR3, GPCR17, FKSG79,GPR174 (LysoPS, LYPSR3), is one of three lysophosphatidyl serine receptors, belonging to the superfamily of G-protein coupled receptors. Lysophospholipids are a diverse group of small, membrane-derived phospholipids that act as extracellular signals1.Activation of GPR174 leads to an increase in cAMP cellular levels, leading to cellular morphological changes, cell to cell adhesion, and delays in cell proliferation2.GPR174 is reported to be a genetic risk locus for Graves disease, an autoimmune disorder that causes hyperthyroidism3 and as a negative regulator of T-cells activation4.

Alternative names: G-protein coupled receptor 174, LYPSR3, GPCR17, FKSG79

Gene ID: 84636

NCBI Accession: NM_032553

UniProt: Q9BXC1