GJC2 antibody (AA 100-111)

Catalog No. ABIN7237684

This anti-GJC2 antibody is a Rabbit Polyclonal antibody detecting GJC2 in WB and IHC. Suitable for Rat.

Quick Overview for GJC2 antibody (AA 100-111) (ABIN7237684)

Target: GJC2 (Gap Junction Protein, gamma 2, 47kDa (GJC2))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GJC2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-GJC2 Antibody

Binding Specificity: AA 100-111

Purpose: A Rabbit Polyclonal antibody to Connexin-47 (GJC2)

Specificity: Intracellular loop.

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,human - identical

Characteristics: Intracellular loop

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)HRLARASEQERR, corresponding to amino acid residues 100 - 111 of rat Connexin-47

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: (ABIN7235562)

Blocking Peptide: (ABIN7235562)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for GJC2

Target: GJC2 (Gap Junction Protein, gamma 2, 47kDa (GJC2))

Alternative Name: GJC2 

Background: CX47, Gap Junction Gamma-2 Protein, Gap Junction Alpha-12 Protein, GJA12, Connexin-46.6,Connexin-47 (Cx47) is a gap junction (GJ) protein expressed in the Central nervous system.It belongs to the connexins family of transmembrane proteins. Proteins that belong to this family form intercellular membrane channels that allow diffusional movement of ions, metabolites, and signaling molecules. The family members are composed of four transmembrane segments, an unstructured C and N cytoplasmic termini, a cytoplasmic loop and two extra-cellular loops1.Connexins oligomerize among the family members to form different structures and Cx47 was shown to form gap junction channels together with Cx43.These voltage gated channels exhibit ionic and chemical rectification, which creates a directional diffusion barrier for the movement of ions and negatively charged molecules from cells expressing Cx47 to those with Cx431.The channel mediates an intercellular communication between oligodendrocytes and between astrocytes. This process is essential to maintain the integrity of myelin and maintaining K+ buffering and nutrient homeostasis in oligodendrocytes, myelin and oligodendrocyte function2,3, and indeed, mutations in human Cx47 cause severe myelin disorders2.Cx43/Cx47 gap junction channels activity was shown to be associated with the development of Multiple Sclerosis and autoimmune demyelination, underscoring a critical role for Cx47 in regulating neuroinflammation4.

Alternative names: CX47, Gap Junction Gamma-2 Protein, Gap Junction Alpha-12 Protein, GJA12, Connexin-46.6

Gene ID: 57165

NCBI Accession: NM_020435

UniProt: Q80XF7