LRRTM2 antibody (Extracellular)

Catalog No. ABIN7237710

This anti-LRRTM2 antibody is a Rabbit Polyclonal antibody detecting LRRTM2 in WB and IHC. Suitable for Mouse.

Quick Overview for LRRTM2 antibody (Extracellular) (ABIN7237710)

Target: LRRTM2 (Leucine Rich Repeat Transmembrane Neuronal 2 (LRRTM2))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LRRTM2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-LRRTM2 Antibody

Binding Specificity: AA 47-61, Extracellular

Purpose: A Rabbit Polyclonal antibody to LRRTM2 (extracellular)

Specificity: Extracellular, N-terminus.

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat,human - identical

Characteristics: Extracellular, N-terminus

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: Peptide CDSQGFHSVPNATDK, corresponding to amino acid residues 47 - 61 of mouse LRRTM2

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:400

Application Dilutions Western blot wb: 1:200-1:400

Comment:

Negative Control: (ABIN7235871)

Blocking Peptide: (ABIN7235871)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for LRRTM2

Target: LRRTM2 (Leucine Rich Repeat Transmembrane Neuronal 2 (LRRTM2))

Alternative Name: LRRTM2 

Background: Leucine-rich repeat transmembrane neuronal protein 2, Leucine-Rich Repeat Neuronal 2 Protein, LRRN2,Leucine-rich repeat transmembrane neuronal proteins (LRRTMs) function as postsynaptic organizers that induce excitatory synapses. There are four related LRRTM genes in mammals, and all are enriched in brain and expressed from postnatal day one or earlier1. LRRTM2 is the most synaptogenic isoform of the family, and its expression is strongly restricted to excitatory synapses in mature neurons. It consists of leucine-rich repeat (LRR) domain in the N-terminal extracellular domain, followed by a single transmembrane domain and a cytoplasmic region contains a PDZ domain binding motif2. LRRTM2 can induce clustering of NMDA receptors, PSD-95 and SynGAP in postsynaptic membrane. PSD-95 function as a scaffold to cluster these postsynaptic proteins. It was found that LRRTM2 deletion in mice leading to dramatically impairs basal synaptic transmission and disrupts long-term potentiation, a prominent form of synaptic plasticity that is critical for learning and memory3. LRRTM2 responsible for the structural linkage mediating nanocolumn alignment of AMPARs, a protein critical for the strength of basal synaptic transmission3.

Alternative names: Leucine-rich repeat transmembrane neuronal protein 2, Leucine-Rich Repeat Neuronal 2 Protein, LRRN2

Gene ID: 107065

NCBI Accession: NM_015564

UniProt: Q8BGA3

Pathways: Synaptic Membrane