MRGPRD antibody (C-Term, Intracellular)

Catalog No. ABIN7886567

The Guinea Pig Polyclonal anti-MRGPRD antibody is suitable to detect MRGPRD in samples from Rat. It has been validated for WB.

Quick Overview for MRGPRD antibody (C-Term, Intracellular) (ABIN7886567)

Target: MRGPRD (MAS-Related GPR, Member D (MRGPRD))

Reactivity: Rat

Host: Guinea Pig

Clonality: Polyclonal

Conjugate: This MRGPRD antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MRGPRD Antibody

Binding Specificity: AA 285-299, C-Term, Intracellular

Purpose: A Guinea Pig Polyclonal Antibody to MRGPRD (GPCR TGR7)

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Mouse - identical It will not recognize MRGPRD from human samples

Characteristics: Intracellular, C-terminus

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)KSHRLQESLGAVLGR, corresponding to amino acid residues 285-299 of rat MRGPRD

Isotype: IgG

Application Details

Application Notes:

WB: 1:500-1:800

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for MRGPRD

Target: MRGPRD (MAS-Related GPR, Member D (MRGPRD))

Alternative Name: MRGPRD

Background:

Synonyms: Mrgd, Mas-related G-protein coupled receptor member D, G-protein coupled receptor TGR7, β-Alanine receptor

Description: Mas-related G-protein coupled receptor member D (MRGPRD) is expressed in the dorsal root ganglia, specifically in non-peptidergic, small-diameter, IB4+ C-fiber neurons that innervate the outer layers of the mammalian skin. They transmit polymodal nociceptive information from the skin epidermis to the substantia gelatinosa of the spinal cord.MRGPRD receptor expressing neurons are essential for the full expression of mechanical nociception. Thus, MRGPRD represents an attractive therapeutic target for the development of agents for treating pain1,2.The Mrg family in mice is comprised of six single-copy genes: MrgD, MrgE, MrgF/RTA, MrgG, MrgH/GPR90, and MAS1. In addition, there are three large subfamilies: MrgA, MrgB,and MrgC3.MRGPRD receptor is activated by β-alanine and is able to inhibit KCNQ/M-type potassium channels and to increase excitability of sensory neurons4,5. MRGPRD has the ability to form heterodimers and is encoded by the TGR7 gene. This receptor is co-expressed with two nociceptors, including purinergic receptor (P2X3) and a vanilloid receptor (VR1)5.

Gene ID: 293648

NCBI Accession: NM_198923

UniProt: Q7TN41