Netrin 3 antibody (Secreted)

Catalog No. ABIN7237732

The Rabbit Polyclonal anti-Netrin 3 antibody has been validated for WB and IHC. It is suitable to detect Netrin 3 in samples from Mouse.

Quick Overview for Netrin 3 antibody (Secreted) (ABIN7237732)

Target: Netrin 3 (Ntn3)

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Netrin 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Netrin 3 Antibody

Binding Specificity: AA 445-458, Secreted

Purpose: A Rabbit Polyclonal antibody to Netrin-3

Specificity: Secreted

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat - identical, Human - 13 out of 14 amino acid residues identical

Characteristics: Secreted

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: Peptide CRPARGSYRISLKK, corresponding to amino acid residues 445 - 458 of mouse Netrin-3

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:1000

Application Dilutions Western blot wb: 1:200-1:400

Comment:

Negative Control: (ABIN7236014)

Blocking Peptide: (ABIN7236014)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for Netrin 3

Target: Netrin 3 (Ntn3)

Alternative Name: NTN3

Background: NTN3, Netrin-2-Like Protein, NTN2L,Netrins are a family of highly conserved proteins responsible for axon guidance and cell movement throughout neural development. Netrins can be divided into secreted netrins (netrin 1,3,4 and 5) and membrane-tethered glycophosphatidylinositol (GPI)-linked netrins (netrin G1 and G2)1.Secreted netrins carry their function via interaction with several receptors that include the deleted in colorectal (DCC) family, and the uncoordinated-5 (UNC5-A through UNC5-D) family2.Netrin-3 was discovered in 1997 using sequence homology searching of netrin-2. Similarly to other netrins, Netrin-3 plays an important role in the development of the nervous system. Netrin-3 structure consist of a laminin-like domain located on the N-terminal, three epidermal growth factors like repeats (EGF), and a C-terminal netrin-like domain (NTR). Mutations in NTN3, the gene encoding netrin-3 was found to be associated with the development of several carcinomas3.

Alternative names: NTN3, Netrin-2-Like Protein, NTN2L

Gene ID: 18209

NCBI Accession: NM_006181

UniProt: Q9R1A3

Pathways: Regulation of Muscle Cell Differentiation