PTAFR antibody (Extracellular, Loop 3) (FITC)

Catalog No. ABIN7237763

The FITC-conjugated Rabbit Polyclonal anti-PTAFR antibody (ABIN7237763) specifically detects PTAFR in FACS and LCI. The antibody is reactive with Mouse samples.

Quick Overview for PTAFR antibody (Extracellular, Loop 3) (FITC) (ABIN7237763)

Target: PTAFR (Platelet-Activating Factor Receptor (PTAFR))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PTAFR antibody is conjugated to FITC

Application: Flow Cytometry (FACS), Live Cell Imaging (LCI)

Product Details anti-PTAFR Antibody (FITC)

Binding Specificity: AA 259-274, Extracellular, Loop 3

Purpose: A Rabbit Polyclonal Antibody to Platelet-activating factor receptor (extracellular) conjugated to the fluorescent dye FITC

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat - identical,Human - 13 out of 16 amino acid residues identical

Characteristics: Extracellular, 3rd loop

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)ELGYQTNFHQAINDAH, corresponding to amino acid residues 259 - 274 of mouse PTAFR 

Isotype: IgG

Application Details

Application Notes:

WB: The optimal concentration should be determined by the user

FC: 2.5-5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7582044)

Blocking Peptide: (ABIN7236256)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 15 μL or 50 μL double distilled water (DDW), depending on the sample size.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for PTAFR

Target: PTAFR (Platelet-Activating Factor Receptor (PTAFR))

Alternative Name: PTAFR

Background:

Synonyms: PTAFR, PAFR

Description: Platelet-activating factor (PAF, 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine) is a biologically active phospholipid mediator that was first described by its ability to cause platelet aggregation and dilation of blood vessels. In addition, PAF acts as a mediator of cell-to-cell communication, inflammation, allergic responses and shock1. PAF acts on PAF receptor (PAFR) a G protein-coupled receptor (GPCR). It consists of 7 transmembrane-spanning domains (TMDs), and two potential N-linked glycosylation sites. PAFR was found to expressed in the lung, neutrophils, monocytes, macrophages, placenta, small intestine, heart, liver, kidney, brain, and spleen. As a results of PAF interaction, PAFR is coupled to Gq protein and consequently activates various downstream signaling pathways2. Experiments in both PAF receptor knockout animals and transgenic animals overexpressing PAF receptors support the pathophysiological role attributed to PAF receptor signaling. For example, Mice model of obstructive nephropathy show that PAFR signaling contributes to a pro-inflammatory environment leading to renal dysfunction and progressive organ failure3. In addition, inhibition of PAFR signaling results in an effective inhibition of experimental tumor growth and metastasis4.

Gene ID: 19204

NCBI Accession: NM_000952

UniProt: Q62035

Pathways: Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin