NHE7 antibody (Extracellular)

Catalog No. ABIN7237818

This anti-NHE7 antibody is a Rabbit Polyclonal antibody detecting NHE7 in WB and IHC. Suitable for Mouse.

Quick Overview for NHE7 antibody (Extracellular) (ABIN7237818)

Target: NHE7 (SLC9A7) (Sodium Hydrogen Exchanger 7 (SLC9A7))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NHE7 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-NHE7 Antibody

Binding Specificity: AA 158-171, Extracellular

Purpose: A Rabbit Polyclonal antibody to Na+/H+ Exchanger 7 (NHE7) (extracellular)

Specificity: Extracellular, 2nd loop.

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat - identical, Human - 13 out of 14 amino acid residues identical

Characteristics: Extracellular, 2nd loop

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)EISPGKINNVEQND, corresponding to amino acid residues 158 - 171 of mouse SLC9A7

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: (ABIN7236882)

Blocking Peptide: (ABIN7236882)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for NHE7

Target: NHE7 (SLC9A7) (Sodium Hydrogen Exchanger 7 (SLC9A7))

Alternative Name: SLC9A7

Background: Sodium/hydrogen exchanger 7, Solute Carrier Family 9 Member A7, SLC9A7, NHE-7,Na+/H+ exchangers (NHEs) also termed solute carrier family 9 (SLC9), are proteins that function as ion transporters. NHEs are present across all kingdoms of life from prokaryotes to eukaryotes and are found to be crucial in preventing intracellular acidification. NHEs are divided into 3 groups SLC9A (NHE1-9), SLC9B (NHA1 and NHA2) and SLC9C according to their cellular location and their function. NHEs regulates cell volume and pH of the cytosol and organelles1.NHE7 is an NHE that is localized predominantly to the trans-Golgi network (TGN) and post-Golgi vesicles. NHE7 differ from other NHEs by its ability to transport both Na+ and K+ via a H+ gradient-dependent manner2. NHE7 mRNA is expressed in wide range of cells but is especially abundant in the brain, skeletal muscle and various secretory tissues3. The predicted membrane topology of NHE7 consist of 12 transmembrane domains (TMDs) and a single N-glycosylation site at position 145. Similarly to the other SLC9A isoforms NHE7 can be assembled as a homodimer. It was recently found that a missense variant in NHE7 associates with multigenerational nonsyndromic intellectual disability (ID)3.

Alternative names: Sodium/hydrogen exchanger 7, Solute Carrier Family 9 Member A7, SLC9A7, NHE-7

Gene ID: 84679

NCBI Accession: NM_032591

UniProt: Q96T83