Tetraspanin 3 antibody (Extracellular)

Catalog No. ABIN7237881

The Rabbit Polyclonal anti-Tetraspanin 3 antibody has been validated for WB, IHC, FACS and LCI. It is suitable to detect Tetraspanin 3 in samples from Human.

Quick Overview for Tetraspanin 3 antibody (Extracellular) (ABIN7237881)

Target: Tetraspanin 3 (TSPAN3)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Tetraspanin 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Product Details anti-Tetraspanin 3 Antibody

Binding Specificity: AA 155-169, Extracellular

Purpose: A Rabbit Polyclonal antibody to Tetraspanin-3 (extracellular)

Specificity: Extracellular, 2nd loop.

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,rat - identical

Characteristics: Extracellular, 2nd loop

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)DWENTDWFKETKNQS, corresponding to amino acid residues 155 - 169 of human Tetraspanin-3

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300

Application Dilutions Western blot wb: 1:200-1:400

Comment:

Negative Control: (ABIN7237377)

Blocking Peptide: (ABIN7237377)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for Tetraspanin 3

Target: Tetraspanin 3 (TSPAN3)

Alternative Name: TSPAN3

Background: TSPAN3, Transmembrane 4 Superfamily Member 8, TM4SF8,Tetraspanin-3, encoded by TSPAN3 gene is a membrane-spanning glycoprotein that has a role in cell migration, proliferation, and interactions between cells and extracellular matrix.As suggested by the family name, TSPANs are categorized by the presence of four trans-membrane domains, of which two are extracellular. The intracellular part includes the C and N terminal domains that can facilitate protein-protein interactions, as well as guide intracellular trafficking. TSPANs have multiple roles In the immune and vascular systems, and they act as organizers of functional microdomains and multi-subunit receptor complexes 1.Tspan3 is highly expressed in the brain and elevated in brains of Alzheimers disease (AD) patients. It was shown to interact with ADAM10, which is a marker for Alzheimer's disease 2. It was also shown to be expressed by dendritic cells (DCs) which suggests that it may be involved in the function of DCs in association with T cell stimulation 3.Its functional diversity and importance for the development and onset of various diseases makes Tetraspanin 3 an appealing research target.

Alternative names: TSPAN3, Transmembrane 4 Superfamily Member 8, TM4SF8

Gene ID: 10099

NCBI Accession: NM_005724

UniProt: O60637