Tetraspanin 7 antibody (Extracellular)

Catalog No. ABIN7237885

This anti-Tetraspanin 7 antibody is a Rabbit Polyclonal antibody detecting Tetraspanin 7 in WB, FACS, IHC and LCI. Suitable for Human.

Quick Overview for Tetraspanin 7 antibody (Extracellular) (ABIN7237885)

Target: Tetraspanin 7 (TSPAN7)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Tetraspanin 7 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), Live Cell Imaging (LCI)

Product Details anti-Tetraspanin 7 Antibody

Binding Specificity: AA 155-169, Extracellular

Purpose: A Rabbit Polyclonal Antibody to Tetraspanin 7 (extracellular)

Specificity: Extracellular, 2nd loop.

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse - 15 out of 16 amino acid residues identical, rat - 14 out of 16 amino acid residues identical

Characteristics: Extracellular, 2nd loop

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)RTYTDAMQTYNGNDER, corresponding to amino acid residues 122 - 137 of human Tetraspanin 7

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300

Application Dilutions Western blot wb: 1:400-1:500

Comment:

Negative Control: (ABIN7237381)

Blocking Peptide: (ABIN7237381)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for Tetraspanin 7

Target: Tetraspanin 7 (TSPAN7)

Alternative Name: TSPAN7

Background: TSPAN7, Transmembrane 4 Superfamily Member 2, TM4SF2, T-Cell Acute Lymphoblastic Leukemia-Associated Antigen 1, TALLA-1, Cell Surface Glycoprotein A15, A15, CD231.,Tetraspanins (Tspans) are a family of four-span transmembrane proteins, known as plasma membrane "master organizers." They form Tspan-enriched microdomains (TEMs or TERMs) through lateral association with one another and other membrane proteins.1Tetraspanins are characterized by four transmembrane domains, two extracellular loops: one small extracellular loop (SEL or EC1) and one larger extracellular loop (LEL or EC2), a short intracellular loop, and two short intracellular tails.1Teatraspanin 7 (TSPAN7) is highly expressed in the brain and was first described as a candidate gene for X-linked intellectual disability, probably through the modulation of surface expression of AMPA and dopamine receptors.2Tetraspanin 7 also has a key role in controlling pancreatic β-cell Ca2+ handling and defining the set-point of insulin secretion through modulation of L-type Cav1.2 and Cav1.3 channels. Furthermore, it has been identified as a target for autoantibodies in Type I diabetes. 3In addition, Tetraspanin 7 is involved in the regulation of HIV virus entry into dendritic cells and several types of cancer progression. 2

Alternative names: TSPAN7, Transmembrane 4 Superfamily Member 2, TM4SF2, T-Cell Acute Lymphoblastic Leukemia-Associated Antigen 1, TALLA-1, Cell Surface Glycoprotein A15, A15, CD231.

Gene ID: 7102

NCBI Accession: NM_004615

UniProt: P41732