TMC1 antibody (Extracellular)

Catalog No. ABIN7237916

This Rabbit Polyclonal antibody specifically detects TMC1 in WB, FACS and LCI. It exhibits reactivity toward Mouse.

Quick Overview for TMC1 antibody (Extracellular) (ABIN7237916)

Target: TMC1 (Transmembrane Channel-Like 1 (TMC1))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TMC1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Product Details anti-TMC1 Antibody

Binding Specificity: Extracellular

Purpose: A Rabbit Polyclonal antibody to TMC1 (extracellular) 

Specificity: Extracellular, 3rd loop. 

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat,human - identical

Characteristics: Extracellular, 3rd loop

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)EHDFPSWMAKILR, corresponding to amino acid residues 679 - 691 of mouse TMC1

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200-1:400

Comment:

Negative Control: (ABIN7237509)

Blocking Peptide: (ABIN7237509)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for TMC1

Target: TMC1 (Transmembrane Channel-Like 1 (TMC1))

Alternative Name: TMC1 

Background: Transmembrane Channel Like 1, Transmembrane cochlear-expressed protein 1,Transmembrane channel-like protein 1 (TMC1) is one of eight mammalian TMC genes. Proteins that belong to this family contain six transmembrane domains with both the C and N termini on the cytoplasmic side of the membrane, as well as a large loop between domains 4 and 5. This multi-pass transmembrane topologic structure suggests roles as a receptor, transporter, channel, or pump1. TMC1 and the closely related TMC2 gene are assembled as dimers2 and are expressed in neurosensory hair cells of the mouse inner ear where they appear to have a role as pore forming units in mechanoelectrical transduction. Mutations of the transmembrane channel-like 1 (TMC1) gene can cause dominant and recessive forms of deafness in humans and mice3 and hence, identification of the molecular functions of Tmc genes is broadly significant because it may provide a handle on the fundamental mechanisms that mediate human hearing and balance4.

Alternative names: Transmembrane Channel Like 1, Transmembrane cochlear-expressed protein 1

Gene ID: 13409

NCBI Accession: NM_138691

UniProt: Q8R4P5

Pathways: Sensory Perception of Sound