TMC1 antibody (Extracellular)

Catalog No. ABIN7237916

Product Details anti-TMC1 Antibody

Target: TMC1 (Transmembrane Channel-Like 1 (TMC1))

Binding Specificity: Extracellular

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TMC1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Live Cell Imaging (LCI)

Purpose: A Rabbit Polyclonal antibody to TMC1 (extracellular) 

Specificity: Extracellular, 3rd loop. 

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat,human - identical

Characteristics: Extracellular, 3rd loop

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)EHDFPSWMAKILR, corresponding to amino acid residues 679 - 691 of mouse TMC1

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200-1:400

Comment:

Negative Control: BLP-TC010

Blocking Peptide: BLP-TC010

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for TMC1

Target: TMC1 (Transmembrane Channel-Like 1 (TMC1))

Alternative Name: TMC1  (TMC1 Products)

Background: Transmembrane Channel Like 1, Transmembrane cochlear-expressed protein 1,Transmembrane channel-like protein 1 (TMC1) is one of eight mammalian TMC genes. Proteins that belong to this family contain six transmembrane domains with both the C and N termini on the cytoplasmic side of the membrane, as well as a large loop between domains 4 and 5. This multi-pass transmembrane topologic structure suggests roles as a receptor, transporter, channel, or pump1. TMC1 and the closely related TMC2 gene are assembled as dimers2 and are expressed in neurosensory hair cells of the mouse inner ear where they appear to have a role as pore forming units in mechanoelectrical transduction. Mutations of the transmembrane channel-like 1 (TMC1) gene can cause dominant and recessive forms of deafness in humans and mice3 and hence, identification of the molecular functions of Tmc genes is broadly significant because it may provide a handle on the fundamental mechanisms that mediate human hearing and balance4.

Alternative names: Transmembrane Channel Like 1, Transmembrane cochlear-expressed protein 1

Gene ID: 13409

NCBI Accession: NM_138691

UniProt: Q8R4P5

Pathways: Sensory Perception of Sound