EAF1 antibody (AA 50-120)

Catalog No. ABIN7266919

This Rabbit Polyclonal antibody specifically detects EAF1 in WB. It exhibits reactivity toward Human.

Quick Overview for EAF1 antibody (AA 50-120) (ABIN7266919)

Target: EAF1 (ELL Associated Factor 1 (EAF1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EAF1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-EAF1 Antibody

Binding Specificity: AA 50-120

Purpose: EAF1 Rabbit pAb

Sequence: LQVGKGDEVT ITLPHIPGST PPMTVFKGNK RPYQKDCVLI INHDTGEYVL EKLSSSIQVK KTRAEGSSKI Q

Cross-Reactivity: Human, Rat

Characteristics: Polyclonal Antibodies

Purification: Affinity purification

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 50-120 of human EAF1 (NP_149074.3).

Isotype: IgG

Application Details

Application Notes: WB,1:500 - 1:2000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide,50 % glycerol, pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for EAF1

Target: EAF1 (ELL Associated Factor 1 (EAF1))

Alternative Name: EAF1

Background: Actin is a key regulator of RNA polymerase (Pol) II-dependent transcription. Positive transcription elongation factor b (P-TEFb), a Cdk9/cyclin T1 heterodimer, has been reported to play a critical role in transcription elongation. However, the relationship between actin and P-TEFb is still not clear. In this study, actin was found to interact with Cdk9, a catalytic subunit of P-TEFb, in elongation complexes. Using immunofluorescence and immunoprecipitation assays, Cdk9 was found to bind to G-actin through the conserved Thr-186 in the T-loop. Overexpression and in vitro kinase assays showed that G-actin promotes P-TEFb-dependent phosphorylation of the Pol II C-terminal domain. An in vitro transcription experiment revealed that the interaction between G-actin and Cdk9 stimulated Pol II transcription elongation. ChIP and immobilized template assays indicated that actin recruited Cdk9 to a transcriptional template in vivo and in vitro. Using cytokine IL-6-inducible p21 gene expression system, we revealed that actin recruited Cdk9 to endogenous gene. Moreover, overexpression of actin and Cdk9 increased histone H3 acetylation and acetylized histone H3 binding to a transcriptional template through the interaction with histone acetyltransferase, p300. Taken together, our results suggested that actin participates in transcription elongation by recruiting Cdk9 for phosphorylation of the Pol II C-terminal domain, and the actin-Cdk9 interaction promotes chromatin remodeling.,EAF1,Epigenetics & Nuclear Signaling,EAF1

Molecular Weight: 17kDa/29kDa

Gene ID: 85403

UniProt: Q96JC9