HCAR2 antibody (AA 250-350)

Catalog No. ABIN7267804

This Rabbit Polyclonal antibody specifically detects HCAR2 in IHC, WB and IF. It exhibits reactivity toward Human.

Quick Overview for HCAR2 antibody (AA 250-350) (ABIN7267804)

Target: HCAR2 (Hydroxycarboxylic Acid Receptor 2 (HCAR2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HCAR2 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-HCAR2 Antibody

Binding Specificity: AA 250-350

Purpose: HCAR2 Rabbit pAb

Sequence: VRIRIFWLLH TSGTQNCEVY RSVDLAFFIT LSFTYMNSML DPVVYYFSSP SFPNFFSTLI NRCLQRKMTG EPDNNRSTSV ELTGDPNKTR GAPEALMANS G

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Polyclonal Antibodies

Purification: Affinity purification

Immunogen: A synthetic peptide corresponding to a sequence within amino acids 250-350 of human HCAR2 (NP_808219.1).

Isotype: IgG

Application Details

Application Notes: WB,1:500 - 1:2000,IHC,1:50 - 1:200,IF,1:50 - 1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide,50 % glycerol, pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for HCAR2

Target: HCAR2 (Hydroxycarboxylic Acid Receptor 2 (HCAR2))

Alternative Name: HCAR2

Background: Acts as a high affinity receptor for both nicotinic acid (also known as niacin and (D-beta-hydroxybutyrate and mediates increased adiponectin secretion and decreased lipolysis through G(i-protein-mediated inhibition of adenylyl cyclase. This pharmacological effect requires nicotinic acid doses that are much higher than those provided by a normal diet. Mediates nicotinic acid-induced apoptosis in mature neutrophils. Receptor activation by nicotinic acid results in reduced cAMP levels which may affect activity of cAMP-dependent protein kinase A and phosphorylation of target proteins, leading to neutrophil apoptosis. The rank order of potency for the displacement of nicotinic acid binding is 5-methyl pyrazole-3-carboxylic acid = pyridine-3-acetic acid > acifran > 5-methyl nicotinic acid = acipimox >> nicotinuric acid = nicotinamide.,HCAR2,GPR109A,HCA2,HM74a,HM74b,NIACR1,PUMAG,Puma-g,Cancer,Tumor suppressors,p53 pathway,Signal Transduction,G protein signaling,G-Protein-Coupled Receptors(GPCR),Cell Biology & Developmental Biology,Apoptosis,Endocrine & Metabolism,Lipid Metabolism,Hydrolysis,Cardiovascular,Lipids,HCAR2

Molecular Weight: 42kDa

Gene ID: 338442

UniProt: Q8TDS4