PTEN antibody

Catalog No. ABIN7755272

This Rabbit Monoclonal antibody specifically detects PTEN in WB, IF, IHC and IP. It exhibits reactivity toward Human.

Quick Overview for PTEN antibody (ABIN7755272)

Target: PTEN (Phosphatase and Tensin Homolog (PTEN))

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This PTEN antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Grade: KO Validated

Product Details anti-PTEN Antibody

Purpose: [KO Validated] PTEN Rabbit mAb

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Monoclonal Antibodies

Purification: Affinity purification

Immunogen: A synthesized peptide derived from human PTEN

Isotype: IgG

Application Details

Application Notes: WB,1:500 - 1:2000,IHC,1:50 - 1:200,IF,1:50 - 1:200,IP,1:50 - 1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide,0.05 % BSA,50 % glycerol, pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for PTEN

Target: PTEN (Phosphatase and Tensin Homolog (PTEN))

Alternative Name: PTEN

Background: This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded by this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. The use of a non-canonical (CUG) upstream initiation site produces a longer isoform that initiates translation with a leucine, and is thought to be preferentially associated with the mitochondrial inner membrane. This longer isoform may help regulate energy metabolism in the mitochondria. A pseudogene of this gene is found on chromosome 9. Alternative splicing and the use of multiple translation start codons results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Feb 2015],10q23del,BZS,CWS1,DEC,GLM2,MHAM,MMAC1,PTEN1,TEP1,PTEN,PTENbeta,Angiogenesis,Apoptosis,Apoptosis_Inhibition of Apoptosis,B Cell Receptor Signaling Pathway,Cancer,Cardiovascular,Cell Biology & Developmental Biology,Cell Cycle,Cytokines,Cytoskeleton_Microtubules,Endocrine & Metabolism,Endocrine and metabolic diseases,Endocrine and metabolic diseases_Diabetes,Endocrine and metabolic diseases_Metabolic disorders,Endocrine and metabolic diseases_Obesity,Epigenetics & Nuclear Signaling,Immunology & Inflammation,Insulin Receptor Signaling Pathway,Kinase,Lipid Metabolism,mTOR Signaling Pathway,PI3K-Akt Signaling Pathway,PI3K-Akt Signaling Pathway_Phosphatase of Akt,Protein phosphorylation,Signal Transduction,Translation Control,Translational Control_Regulation of eIF4 and p70 S6 Kinase,Tumor suppressors,PTEN

Molecular Weight: 54kDa

Gene ID: 5728

UniProt: P60484

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Inositol Metabolic Process, Synaptic Membrane, Regulation of Cell Size, Autophagy, Platelet-derived growth Factor Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling