SARS-Coronavirus Nonstructural Protein 8 (SARS-CoV NSP8) antibody

Catalog No. ABIN7270150

This Rabbit Monoclonal antibody specifically detects in WB and IF. It exhibits reactivity toward SARS Coronavirus-2 (SARS-CoV-2).

Quick Overview for SARS-Coronavirus Nonstructural Protein 8 (SARS-CoV NSP8) antibody (ABIN7270150)

Target: SARS-Coronavirus Nonstructural Protein 8 (SARS-CoV NSP8)

Reactivity: SARS Coronavirus-2 (SARS-CoV-2)

Host: Rabbit

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details

Purpose: SARS-CoV-2 3CLpro Rabbit mAb

Cross-Reactivity: SARS Coronavirus-2 (SARS-CoV-2)

Characteristics: Monoclonal Antibodies

Purification: Affinity purification

Immunogen: Recombinant fusion protein of SARS-CoV-2 3CLpro.

Isotype: IgG

Application Details

Application Notes: WB,1:500 - 1:2000,IF,1:50 - 1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide,50 % glycerol, pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details

Target: SARS-Coronavirus Nonstructural Protein 8 (SARS-CoV NSP8)

Alternative Name: 3CLpro

Target Type: Viral Protein

Background: [Spike protein S1]: attaches the virion to the cell membrane by interacting with host receptor, initiating the infection. Binding to human ACE2 receptor and internalization of the virus into the endosomes of the host cell induces conformational changes in the Spike glycoprotein. Binding to host NRP1 and NRP2 via C-terminal polybasic sequence enhances virion entry into host cell. This interaction may explain virus tropism of human olfactory epithelium cells, which express high level of NRP1 and NRP2 but low level of ACE2. The stalk domain of S contains three hinges, giving the head unexpected orientational freedom. Uses human TMPRSS2 for priming in human lung cells which is an essential step for viral entry. Can be alternatively processed by host furin. Proteolysis by cathepsin CTSL may unmask the fusion peptide of S2 and activate membranes fusion within endosomes.,3CLpro

Gene ID: 43740578

UniProt: P0DTC2