SMAD4 antibody

Catalog No. ABIN7270340

The Rabbit Monoclonal anti-SMAD4 antibody has been validated for WB, IHC and IP. It is suitable to detect SMAD4 in samples from Human.

Quick Overview for SMAD4 antibody (ABIN7270340)

Target: SMAD4 (SMAD Family Member 4 (SMAD4))

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This SMAD4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Grade: KO Validated

Product Details anti-SMAD4 Antibody

Purpose: [KO Validated] Smad4 Rabbit mAb

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Monoclonal Antibodies

Purification: Affinity purification

Immunogen: Recombinant protein of human Smad4.

Isotype: IgG

Application Details

Application Notes: WB,1:500 - 1:2000,IHC,1:50 - 1:200,IP,1:50 - 1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide,0.05 % BSA,50 % glycerol, pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for SMAD4

Target: SMAD4 (SMAD Family Member 4 (SMAD4))

Alternative Name: SMAD4

Background: This gene encodes a member of the Smad family of signal transduction proteins. Smad proteins are phosphorylated and activated by transmembrane serine-threonine receptor kinases in response to TGF-beta signaling. The product of this gene forms homomeric complexes and heteromeric complexes with other activated Smad proteins, which then accumulate in the nucleus and regulate the transcription of target genes. This protein binds to DNA and recognizes an 8-bp palindromic sequence (GTCTAGAC) called the Smad-binding element (SBE). The Smad proteins are subject to complex regulation by post-translational modifications. Mutations or deletions in this gene have been shown to result in pancreatic cancer, juvenile polyposis syndrome, and hereditary hemorrhagic telangiectasia syndrome. [provided by RefSeq, Oct 2009],DPC4,JIP,MADH4,MYHRS,Smad4,SMAD4,Apoptosis,Cancer,Cardiovascular,Cell Biology & Developmental Biology,Cell Cycle,Endocrine & Metabolism,Epigenetics & Nuclear Signaling,ESC Pluripotency and Differentiation,G1/S Checkpoint,Heart,Heart_Cardiogenesis,Heart_Hypertrophy,Hypoxia,MAPK-JNK Signaling Pathway,Signal Transduction,Stem Cells,TGF-b-Smad Signaling Pathway,TGF-b-Smad Signaling Pathway_SMADs,Transcription Factors,Tumor suppressors,SMAD4

Molecular Weight: 65kDa

Gene ID: 4089

UniProt: Q13485

Pathways: Cell Division Cycle, Chromatin Binding, Autophagy