Spt6 antibody (AA 1500-1726)

Catalog No. ABIN7270645

This Rabbit Polyclonal antibody specifically detects Spt6 in WB, IP and IF. It exhibits reactivity toward Human.

Quick Overview for Spt6 antibody (AA 1500-1726) (ABIN7270645)

Target: Spt6 (SUPT6H) (Suppressor of Ty 6 Homolog (SUPT6H))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Spt6 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF)

Product Details anti-Spt6 Antibody

Binding Specificity: AA 1500-1726

Purpose: SUPT6H Rabbit pAb

Sequence: IFPTVNGLFR WFKDHYQDPV PGITPSSSSR TRTPASINAT PANINLADLT RAVNALPQNM TSQMFSAIAA VTGQGQNPNA TPAQWASSQY GYGGSGGGSS AYHVFPTPAQ QPVATPLMTP SYSYTTPSQP ITTPQYHQLQ ASTTPQSAQA QPQPSSSSRQ RQQQPKSNSH AAIDWGKMAE QWLQEKEAER RKQKQRLTPR PSPSPMIEST PMSIAGDATP LLDEMDR

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Polyclonal Antibodies

Purification: Affinity purification

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1500-1726 of human SUPT6H (NP_003161.2).

Isotype: IgG

Application Details

Application Notes: WB,1:500 - 1:2000,IF,1:50 - 1:200,IP,1:50 - 1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide,50 % glycerol, pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for Spt6

Target: Spt6 (SUPT6H) (Suppressor of Ty 6 Homolog (SUPT6H))

Alternative Name: SUPT6H

Background: Transcription elongation factor which binds histone H3 and plays a key role in the regulation of transcription elongation and mRNA processing. Enhances the transcription elongation by RNA polymerase II (RNAPII and is also required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. Besides chaperoning histones in transcription, acts to transport and splice mRNA by forming a complex with IWS1 and the C-terminal domain (CTD of the RNAPII subunit RPB1 (POLR2A. The SUPT6H:IWS1:CTD complex recruits mRNA export factors (ALYREF/THOC4, EXOSC10 as well as histone modifying enzymes (such as SETD2, to ensure proper mRNA splicing, efficient mRNA export and elongation-coupled H3K36 methylation, a signature chromatin mark of active transcription. SUPT6H via its association with SETD1A, regulates both class-switch recombination and somatic hypermutation through formation of H3K4me3 epigenetic marks on activation-induced cytidine deaminase (AICDA target loci. Promotes the activation of the myogenic gene program by entailing erasure of the repressive H3K27me3 epigenetic mark through stabilization of the chromatin interaction of the H3K27 demethylase KDM6A.,SUPT6H,SPT6,SPT6H,emb-5,SPT6 homolog,Epigenetics & Nuclear Signaling,RNA Binding,Chromatin Remodeling,SUPT6H

Molecular Weight: 61kDa/84kDa/199kDa

Gene ID: 6830

UniProt: Q7KZ85

Pathways: Regulation of Leukocyte Mediated Immunity, Regulation of Muscle Cell Differentiation, Production of Molecular Mediator of Immune Response