Topoisomerase I antibody

Catalog No. ABIN7270850

The Rabbit Monoclonal anti-Topoisomerase I antibody has been validated for WB, IHC, IP and IF. It is suitable to detect Topoisomerase I in samples from Human.

Quick Overview for Topoisomerase I antibody (ABIN7270850)

Target: Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Topoisomerase I antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF)

Product Details anti-Topoisomerase I Antibody

Purpose: DNA topoisomerase I (TOP1) Rabbit mAb

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Monoclonal Antibodies

Purification: Affinity purification

Immunogen: A synthesized peptide derived from human DNA topoisomerase I (DNA topoisomerase I (TOP1))

Isotype: IgG

Application Details

Application Notes: WB,1:500 - 1:2000,IHC,1:50 - 1:200,IF,1:50 - 1:200,IP,1:50 - 1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide,0.05 % BSA,50 % glycerol, pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for Topoisomerase I

Target: Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))

Alternative Name: TOP1

Background: This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22. [provided by RefSeq, Jul 2008],TOPI,Cancer,Drug resistance,Drug resistance_Topoisomerases,Epigenetics & Nuclear Signaling,RNA Binding,TOP1

Molecular Weight: 91kDa

Gene ID: 7150

UniProt: P11387

Pathways: Caspase Cascade in Apoptosis, Stem Cell Maintenance