SUPT5H antibody

Catalog No. ABIN7270878

The Rabbit Monoclonal anti-SUPT5H antibody (ABIN7270878) specifically detects SUPT5H in WB, IHC and IF. The antibody is reactive with Human samples.

Quick Overview for SUPT5H antibody (ABIN7270878)

Target: SUPT5H (Transcription Elongation Factor SPT5 (SUPT5H))

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This SUPT5H antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-SUPT5H Antibody

Purpose: SUPT5H/SPT5 Rabbit mAb

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Monoclonal Antibodies

Purification: Affinity purification

Immunogen: Recombinant protein of human SUPT5H/SPT5.

Isotype: IgG

Application Details

Application Notes: WB,1:500 - 1:2000,IHC,1:50 - 1:200,IF,1:50 - 1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide,0.05 % BSA,50 % glycerol, pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for SUPT5H

Target: SUPT5H (Transcription Elongation Factor SPT5 (SUPT5H))

Alternative Name: SUPT5H

Background: Component of the DRB sensitivity-inducing factor complex (DSIF complex, which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3' terminus and may allow repeated attempts at transcription through natural pause sites. DSIF can also positively regulate transcriptional elongation and is required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. DSIF acts to suppress transcriptional pausing in transcripts derived from the HIV-1 LTR and blocks premature release of HIV-1 transcripts at terminator sequences.,SUPT5H, SPT5, SPT5H, Tat-CT1, transcription elongation factor SPT5,Epigenetics & Nuclear Signaling,Transcription Factors,RNA Binding,Cell Biology & Developmental Biology,Apoptosis,SUPT5H

Molecular Weight: 155kDa

Gene ID: 6829

UniProt: O00267